中医药学报2017，Vol.45Issue(5)：17-20,4.
HPLC测定柴胡加龙骨牡蛎汤改善睡眠有效部位中5种化学成分的含量
Determination of Five Chemical Components of Chaihu Jia Longgu Muli Decoction for Improving Sleep by HPLC
摘要
Abstract
Objective:To establish a method for content determination of baicalin,wogonoside,baicalein,wogonin,and cinnamic acid in Chaihu Jia Longgu Muli decoction by HPLC.Methods:The chromatographic separation was performed using a Diamonsil C18 column(250 mm ×4.6 mm,5 μm) with 25℃ column temperature.The mobile phase was composed of a gradient elution of acetonitrile and 0.5 ％ phosphoric acid at flow rate of 1.0mL · min.Results:The average contents of baicalin,wogonoside,baicalein,wogonin,and cinnamic acid were respectively 1.061％,0.672％,1.168％,0.299％,and 1.424％.The precision,reproducibility and sample recovery of RSD was lower than 3.0％.Conclusion:The method with simple process,good accuracyand reproducibility,is shown to be suitable for the quality control of the effective components of Chaihu Jia Longgu Muli decoction for improving sleep.关键词
HPLC/柴胡加龙骨牡蛎汤/化学成分/睡眠Key words
HPLC/Chaihu Jia Longgu Muli decoction/Chemical components/Sleep分类
医药卫生引用本文复制引用
黄莉莉,耿一玄,许丹丹,杜纳纳..HPLC测定柴胡加龙骨牡蛎汤改善睡眠有效部位中5种化学成分的含量[J].中医药学报,2017,45(5):17-20,4.基金项目
黑龙江省普通高等学校青年学术骨干支持计划(1254G054) （1254G054）
黑龙江省博士后科研启动金(LBH-Q13161) （LBH-Q13161）
黑龙江中医药大学校科研基金资助(2012bs01) （2012bs01）
黑龙江中医药大学”优秀创新人才支持计划”(2012RCD02) （2012RCD02）
国家自然科学基金青年基金(30801528) （30801528）
