中医药学报2017，Vol.45Issue(5)：31-35,5.
花旗泽仁对胰岛素抵抗大鼠肌肉组织中CaSR基因、蛋白表达及AKT活性的影响
Influence of Huaqizeren on the Levels of CaSR Expression and AKT Activity in Muscle Tissues of T2DM Rats with IR
摘要
Abstract
Objective:To explore the mechanism of Huaqizeren(HQZR) in the treatment of insulin resistance(IR) by investigating the effect of HQZR on calcium receptor(CaSR) gene,its protein expression and AKT activity in the muscle tissues of T2DM Rats with IR.Methods:The IR model was established with compound fat emulsion diet for 4 weeks and small-dose STZ injection in male Wistar rats.The successful model rats were randomly divided into the model group,the HQZR group,and the positive control group,which was given Rosiglitazone.FBG(fasting blood glucose) and FINS (fasting insulin) were tested,and ISI (insulin sensitivity index) was calculated.The expressions of CaSR mRNA,CaSR protein and phosphorylated AKT(Ser473 and Thr308) in the muscles were detected with qRT-PCR and Western blot techniques.Results:IR models were successfully established.After HQZR treatment,the levels of FBG and FINS were significantly increased with decreased ISI compared to the model group (P ＜ 0.01).The expressions of CaSR mRNA,CaSR protein and phosphorylated AKT were remarkably increased (P ＜ 0.01,P ＜ 0.05).Conclusion:HQZR may improve IR by regulating CaSR mRNA,protein CaSR and phosphorylated AKT.关键词
花旗泽仁/胰岛素抵抗/钙敏感受体/AKTKey words
HQZR/IR/CaSR/AKT分类
医药卫生引用本文复制引用
黄启晶,李璐,赵聪,郑义,阚玉娜,刘笑男,刘萍,韩东卫,葛鹏玲..花旗泽仁对胰岛素抵抗大鼠肌肉组织中CaSR基因、蛋白表达及AKT活性的影响[J].中医药学报,2017,45(5):31-35,5.基金项目
国家科技重大专项(2012ZX09103201-018) （2012ZX09103201-018）
国家自然科学基金项目(81273650) （81273650）
黑龙江省自然科学基金项目(LC2011C03) （LC2011C03）
黑龙江中医药大学”优秀创新人才支持计划”(No.2012RCD19) （No.2012RCD19）
黑龙江省博士后科研启动基金项目(No.LBH-Q15136) （No.LBH-Q15136）
哈尔滨市科技创新人才研究专项资金项目(No.2016RAXXJ100) （No.2016RAXXJ100）
黑龙江中医药大学科研基金项目(No.201515) （No.201515）
黑龙江省中医药科研项目(No.ZHY16-093) （No.ZHY16-093）
