中医药学报2017，Vol.45Issue(5)：40-44,5.
隐丹参酮调节PCOS模型大鼠卵巢颗粒细胞生殖内分泌机能的机制研究
Mechanism of Cryptotanshinone Regulating Reproductive Endocrine Dysfunction in the Granulosa Cells of PCOS Rats
摘要
Abstract
Objective:To explore the changing tendency of PI3K pathway in the granulosa cells of PCOS model rats,and to discuss the possible mechanism of cryptotanshinone(CT) regulating granulosa cells in PCOS rats.Methods:Granulosa cells were divided into the blank control group(normal rats),the PCOS model group(DHEA intervened rats),CT group (DHEA intervened rats + 300 nM CT for 24 h).Apoptosis ratio,hormone level in cell-culture medium,the expression of steroid hormone synthetase,glucose uptake ability,and expression of PI3 K pathway were examined after in vitro culture and medications.Results:The abnormalities of cells' glucose uptake and steroid hormone level were adjusted by CT in PCOS rats.CT also decreased expressions of STAR and CYP17 with the activation of PI3K pathway.Conclusion:The mechanism of CT treating abnormal reproductive function in PCOS rats may be related to the activation of PI3K path关键词
PCOS/PI3K/颗粒细胞/隐丹参酮Key words
PCOS/PI3K/Granulosa cell/Cryptotanshinone分类
医药卫生引用本文复制引用
匡洪影,马珂昕,李威,陈静,孙荣,张跃辉..隐丹参酮调节PCOS模型大鼠卵巢颗粒细胞生殖内分泌机能的机制研究[J].中医药学报,2017,45(5):40-44,5.基金项目
国家自然科学基金青年基金(81503610) （81503610）
黑龙江省自然科学基金面上项目(H2016083) （H2016083）
哈尔滨市科技创新人才研究专项基金(RC2015QN00309) （RC2015QN00309）
黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2016076) （UNPYSCT-2016076）
黑龙江省留学回国人员择优资助(LBH-Z11006) （LBH-Z11006）
