中医药学报2017，Vol.45Issue(5)：52-56,5.
头针联合川芎嗪对脑缺血再灌注模型大鼠脑组织MyD88和皮层神经元损伤的影响
Impact of Scalp-acupuncture Combined with TMZ on MyD88 Expression and Cortical Neuron Injury in Rats with Cerebral Ischemia Reperfusion
摘要
Abstract
Objective:To observe the effects of scalp-acupuncture combined with Tetramethylpyrazine(TMP) on neurological function,cerebral infarction size,cortical neuron injury and MyD88 expression in rats with cerebral ischemia reperfusion,and to explore the possible mechanism of its brain protection.Methods:165 rats were randomly divided into sham operation group (group A),model group (group B),scalp-acupuncture group (group C),TMP group (group D),and scalp-acupuncture + TMP group (group E).According to different reperfusion time,each group was divided into three sub-groups,i.e.IR1d(n =13),IR3d (n =10),and IR7d(n =10).The models were prepared by using the modified Zea Longa method of 2 h reperfusion(MCAO/R) in right medial cerebral artery.The blood flow of medial cerebral artery was not interrupted in group A;the rats in group C and E group were intervened with Baihui-Qubin scalp-acupuncture on the trouble side;and the rats in group D and group E were given 20 mg/kg TMP once daily.Scores of neural function at corresponding time points were evaluated;size of cerebral infarction was detected with TTC staining in 1 d rats;injury of cortical neurons and inflammatory cell infiltration were observed with HE staining;and expression of MyD88 was detected by immunohistoy chemistry and Western blot method.Results:The degrees of neurological impairment in group E were significantly less than those of the other groups respectively after 1 d,3 d and 7d reperfusion(P ＜0.05).After 1 d reperfusion,the size of cerebral infarction was the smallest in group E,and there was a statistical difference compared to the other groups (P ＜ 0.05).The injury degrees of ischemic and inflammatory cell infiltration group E were significantly lower than those in the other four groups,also the levels of MyD88 positive cells and the relative protein in the ischemic lateral brain tissue were significantly lower than those of the other four groups (P ＜ 0.05).Conclusion:The therapy of scalp-acupuncture with TMP can significantly improve nerve function in the MCAO/R rats,reduce infarction size,decrease cortex neuron injury and inflammatory cells infiltration;it has the action of brain protection with the possible mechanism of inhibiting MyD88 expression and reducing inflammatory cascade reaction after brain ischemia reperfusion.关键词
头针/川芎嗪/脑缺血再灌注损伤/髓样分化因子88Key words
Scalp-acupuncture/Tetramethylpyrazine/Cerebral ischemia reperfusion injury/MyD88分类
医药卫生引用本文复制引用
吴明娟,孙晓伟,高延宏,卢金荣,赵岩,李锐,辛淑梅,程康陆..头针联合川芎嗪对脑缺血再灌注模型大鼠脑组织MyD88和皮层神经元损伤的影响[J].中医药学报,2017,45(5):52-56,5.基金项目
黑龙江省自然科学基金(H2016045) （H2016045）
黑龙江省博士后资助项目(LHB-Z12248) （LHB-Z12248）
