癌变·畸变·突变2018，Vol.30Issue(1)：20-24,5.DOI:10.3969/j.issn.1004-616x.2018.01.004
大蒜素对顺铂肾毒性的保护作用
Protective effects of allicin on cisplatin-induced toxicity in HEK293 kidney cells
摘要
Abstract
OBJECTIVE:To investigate the protective effect of allicin on cisplatin (DDP)-induced oxidative damage in HEK293 cells.METHODS:HEK293 cells were cultured in vitro and organized into different experimental groups:control,DDP treatment,allicin treatment,DDP+allicin treatment.Cell survival and expression of cytotoxicity were determined by using the MTT method.Superoxide dismutase (SOD) activity was examined by the xanthine oxidase method,malondialdehyde (MDA) content by the thiobarbituric acid method and glutathione (GSH) content by the nitrobenzoic acid method.RESULTS:Cell survival rate decreased gradually when treated with DDP alone,and this effect was dose-dependent.After pretreatment with catechin and then with DDP (20 mg/L),the cell survival rate improved significantly (P＜0.01).Allicin treatment significantly increased the intracellular MDA content,but decreased the SOD activity and GSH level compared with that in the DDP control group (P＜0.01).CONCLUSION:Allicin can antagonize DDP-induced oxidative damage in HEK293 cells and has obvious protective effects on DDP-induced toxicity in kidney cells.关键词
大蒜素/顺铂/肾毒性/抗氧化Key words
allicin/cisplatin/nephrotoxicity/antioxidant分类
医药卫生引用本文复制引用
韩何丹,王海,高丽萍..大蒜素对顺铂肾毒性的保护作用[J].癌变·畸变·突变,2018,30(1):20-24,5.基金项目
北京市自然科学基金资助项目(7163211) （7163211）
