癌变·畸变·突变2018，Vol.30Issue(1)：25-30,6.DOI:10.3969/j.issn.1004-616x.2018.01.005
大豆异黄酮对阿特拉津损伤SH-SY5Y细胞的保护作用及其机制
Mechanism of isoflavones protection of SH-SY5Y cells from atrazine
摘要
Abstract
OBJECTIVE:We tested the ability of five candidate compounds-isoflavones,resveratrol,quereetin,eurcumin,and green tea polyphenols-to protect SH-SY5Y cells from atrazine (ATR) via in vitro experiments.METHODS:The cells were incubated for 24 h in the presence of various doses (5,10,20,50,and 100 μmol/L) of isoflavones,resveratrol,quercetin,curcumin,or green tea polyphenols.Cells were then cultured for 24 h with 250 μmol/L ATR.At the indicated time after treatment,the CCK-8 method was used to test for cell viability.Apoptosis was detected by using Annexin V-FITC and PI double dye,oxygen radical level was detected by using DCFH-DA method,and the protein expression of tyrosine hydroxylase (TH) by using Western blot.RESULTS:Five plant compounds showed significant anti-ATR effect at low doses,isoflavones 5 μmol/L,resveratrol 5 μmol/L,quercetin 50 μmol/L,curcumin 5 μmol/L and green tea polyphenols 20 μmol/L.Compared with other four plant compounds,the effect of isoflavones antiATR injury on SH-SY5Y was significant,especially in 5 μmol/L (P＜O.05).And isoflavones (5 μmol/L) could significantly reduce the level of ROS and increase the expression of TH (P＜O.05).CONCLUSION:Isoflavones could protect SH-SY5Y cells from ATR by reducing intracellular ROS levels,increasing the expression of TH and cell viability to inhibit cell apoptosis.关键词
大豆异黄酮/阿特拉津/细胞凋亡/氧自由基/酪氨酸氢化酶Key words
isoflavones/atrazine/cell apoptosis/oxygen radical/tyrosine hydroxylase分类
医药卫生引用本文复制引用
李鹏,马焜,吴浩宇,吴艳萍,李百祥..大豆异黄酮对阿特拉津损伤SH-SY5Y细胞的保护作用及其机制[J].癌变·畸变·突变,2018,30(1):25-30,6.基金项目
国家自然科学基金(81273109) （81273109）
