癌变·畸变·突变2018，Vol.30Issue(1)：37-41,5.DOI:10.3969/j.issn.1004-616x.2018.01.007
异欧前胡素对人表皮黑素细胞酪氨酸酶和Rab27a的调节作用
Effects of isoimperaterin on regulation of tyrosinase and Rab27a in human epidermal melanocytes
摘要
Abstract
OBJECTIVE:To investigate the effects of isoimperaterin on regulation of tyrosinase and Rab27a in cultured human epidermal melanocytes.METHODS:Human epidermal melanocytes were isolated and cultured.The cells were randomly divided into control and experimental groups.Melanocytes of the control group were cultured with M-254 culture medium.Cells of the experimental group were cultured with M-254 culture medium containing 25 μmol/L isoimperaterin.The cells were cultured for 48 h and observed using inverted microscopy.Cell vitality was assayed using MTT method.The expression of tyrosinase and transfer-related protein Rab27a were analyzed by western blotting.The activity of tyrosinase and the content of melanin were measured by L-dopa oxidation and NaOH method,respectively.RESULTS:Compared with the control group,the dendrites of melanocytes of experimental group were elongated after treatment with 25 μmol/L isoimperaterin for 48 h.Melanocyte vitality was reduced by 17.2％ (P＜0.05).Expression of tyrosinase was decreased by 30.3％ (P＜0.01) and Rab27a was increased by 31.9％ (P＜0.05).The activity of tyrosinase was reduced by 25.6％ (P＜0.05).Melanin content was decreased by 30.9％ (P＜0.01).CONCLUSION:25 μmol/L isoimperaterin elongated melanocytic dendrites but inhibited melanocyte vitality.It also inhibited the expression and activity of tyrosinase and promoted the expression of transfer-related protein Rab27a to reduce the content of melanin in cultured human epidermal melanocytes.关键词
黑素细胞/异欧前胡素/酪氨酸酶/Rab27a/黑素Key words
melanocyte/isoimperatorin/tyrosinase/Rab27a/melanin分类
医药卫生引用本文复制引用
孙贝蕾,郑宇琼,林玲,陈玮莹,蔡尔慧,陈玮芳..异欧前胡素对人表皮黑素细胞酪氨酸酶和Rab27a的调节作用[J].癌变·畸变·突变,2018,30(1):37-41,5.基金项目
广东省中医药局项目(20142082) （20142082）
