癌变·畸变·突变2018，Vol.30Issue(1)：42-46,5.DOI:10.3969/j.issn.1004-616x.2018.01.008
辐射诱发大鼠体内旁效应中肺及肾组织凋亡相关基因mRNA的表达变化
Expression of apoptosis-related genes in lung and kidney tissues of rats with radiation-induced bystander effects
摘要
Abstract
OBJECTIVE:To investigate expression of apoptosis-related genes during different dose of X-rayinduced bystander effects in rats.METHODS:70 male Wistar rats were randomly divided into the normal control group and the different irradiation groups.For the latter groups,the right lungs of the rats were radiated with 2,4,8 Gy X-rays.During irradiation,the rest of the body was covered with lead shielding.At 6,24 and 48 h respectively after radiation,7 rats of each radiation group were executed.Variations in mRNA expression of Cytochrome C,Caspase-9,Caspase-3,Bax and Bcl-2 in the right lung,left lung and left kidney tissue were detected with quantitative polymerase chain reaction (qPCR).RESULTS:The mRNA expressions of Cytochrome C,Caspase-9 and Caspase-3 in the right lung,left lung and left kidney tissue of the irradiated groups were up-regulated at all time points.However,the mRNA expressions of Bcl-2 were down-regulated at all time points in the right and left lungs.The observed changes were significantly different in comparison with the normal control group (P＜0.05 or P＜0.01).In the right lung,expressions of Bax mRNA were significantly increased in all irradiated groups except the 8 Gy dose group at 48 h (P＜0.05 or P＜0.01).In the left lung,expressions of Bax mRNA were significantly increased in each radiation group (P＜0.01).In the left kidney,expressions of Bax mRNA were significantly increased in all irradiated groups except the 2 Gy dose group at 6 h.Compared with the normal control group,the differences were statistically significant (P＜0.01).Expressions of Bcl-2 mRNA were decreased at all time points after irradiation except the 8 Gy dose group at 6 h and the difference was statistically significant compared with the normal control group (P＜0.05 or P＜0.01).CONCLUSION:After the right lungs of the rats were irradiated by different doses of X-rays,expressions of Cytochrome C,Caspase-9,Caspase-3 and Bax in the right lung tissue were up-regulated.While expressions of Bcl-2 were down-regulated.The left lung and left kidney tissues that were not irradiated also responded to the X-rays.And the radiation-induced bystander effects were observed in vivo.Between every radiation dose and every time point,there were no apparently different effects.关键词
辐射旁效应/凋亡相关基因/肺肾组织/mRNA表达Key words
radiation induced bystander effect/apoptosis-related genes/lung and kidney tissue/mRNA expression分类
医药卫生引用本文复制引用
张朝宁,李金田,刘永琦,蔺兴遥..辐射诱发大鼠体内旁效应中肺及肾组织凋亡相关基因mRNA的表达变化[J].癌变·畸变·突变,2018,30(1):42-46,5.基金项目
国家自然科学基金项目(81460695) （81460695）
