癌变·畸变·突变2018，Vol.30Issue(1)：47-51,57,6.DOI:10.3969/j.issn.1004-616x.2018.01.009
HIF-1α高表达对苯代谢物诱导K562细胞毒性的影响
Relationship between expression of HIF-1α and cytotoxicity from exposure to benzene metabolites on K562 cells
摘要
Abstract
OBJECTIVE:To study the effects of expression of HIF-1 α on cytotoxicity of K562 cells induced by 1,4-benzoquinone (1,4-BQ),hydroquinone (HQ) and phenol.METHODS:The control K562 cells (K-c) and K562 cells with high expression of HIF-1α (K-h) were treated with different concentrations of 1,4-BQ (0,10,20,40,80 μmol/L),HQ (0,10,20,40,80 μmol/L) and phenol (0,1,1.5,2,2.5,5 mmo]/L) for 24 h.Cell proliferation was detected by MTI assay,apoptosis was detected by flow cytometry with PI/FITC Annxein V double staining,and cell cycle was detected by propidium iodide combined with flow cytometry.RESULTS:The MTT results revealed that the relative proliferation rate of K-c cells and K-h cells showed a dose-related decrease after treating with 1,4-BQ (P＜0.05).The relative proliferation rate of K-h was higher than control K-c (P＜0.05) in 80 μ mol/L 1,4-BQ.After treating with HQ and phenol at higher concentrations,K-c exhibited proliferation inhibition,while no significant proliferation inhibition was observed in K-h.Flow cytometry results revealed that the apoptosis rate increased in K-c and K-h (P＜0.05) and showed a dose-response relationship after exposuring to 1,4-BQ for 24 h (P＜0.05).Moreover,the apoptosis rate of K-hwas lower than K-c (P＜0.05) with 20 μmol/L 1,4-BQ.After exposure to HQ and phenol,K-c and K-h demonstrated no significant differences compared with control group,K-h was not significantly different from K-c.The results of cell cycle analysis demonstrated K-c were arrested in G0/G1 phase or S-phase after exposure to 1,4-BQ,HQ and phenol.CONCLUSION:Among benzene metabolites,1,4-BQ was the most cytotoxic,followed by HQ and phenol.High expression of HIF-1α reduced the cytotoxicity of K562 cell caused by benzene metabolites,and HIF-1α might work in regulating cell proliferation,apoptosis through ceil cycle control.关键词
HIF-1α/苯代谢物/细胞增殖/细胞凋亡/细胞周期Key words
HIF-1α/benzene metabolites/proliferation/apoptosis/cell cycle分类
医药卫生引用本文复制引用
孙凤霞,孟醒,孙蓉丽,张娟,尹立红,浦跃朴..HIF-1α高表达对苯代谢物诱导K562细胞毒性的影响[J].癌变·畸变·突变,2018,30(1):47-51,57,6.基金项目
国家自然科学基金资助项目(81573189,81730087) （81573189,81730087）
