北京林业大学学报2018，Vol.40Issue(2)：22-30,9.DOI:10.13332/j.1000-1522.20170245
杨树PsnGA20ox1过表达对烟草叶片发育的影响
Effects of poplar PsnGA20ox1 overexpression on leaf development of tobacco
摘要
Abstract
[Objective] GA20ox is one of the genes coding key rate-limiting enzymes, GA20-oxidases (GA20ox), for biosynthesis of bioactive gibberellins ( GA) which involve in many biology process of plant growth and development. At present, it is still not clear that the biological function of GAs in plant leaf development. The aim of this research is to reveal the roles of PsnGA20ox1 in the leaves of overexpression tobacco. [ Method ] In this study, we systematically analyzed the leaf size, ultrastructure, biomass, chlorophyll and bioactivity GAs contents, and gene expression level involved in cell division and stomatal formation of PsnGA20ox1 overexpression tobacco and wild-type plants. [Result] Our results revealed that the PsnGA20ox1 overexpression tobacco displayed the enlarged leaf size, enhanced chlorophyll and GA4 contents, and decreased epidermal cell size. In addition, the thickness of palisade and spongy parenchyma, the sizes of epidermal cell, the numbers of stomatal and epidermal cell, and photosynthesis and leaf biomass productivity of PsnGA20ox1 overexpression tobacco were notably increased compared with wild-type tobacco, respectively. Furthermore, the expression levels of genes involved in cell division and stomatal formation in PsnGA20ox1 overexpression tobacco altered notably,and the variation tendency was in accordance with the alternations of leaf trait. [Conclusion] The results demonstrated that the overexpression of PsnGA20ox1 had effect on the structure and development of leaf in the transgenic tobacco, which is beneficial to the accumulation of biomass of leaf.关键词
杨树/PsnGA20ox1/过表达/叶片/发育Key words
poplar/PsnGA20ox1/overexpression/leaf/development分类
农业科技引用本文复制引用
刘露露,鲁婷婷,王爽,李美靓,赵双菁,刘莹莹,魏志刚..杨树PsnGA20ox1过表达对烟草叶片发育的影响[J].北京林业大学学报,2018,40(2):22-30,9.基金项目
国家自然科学基金项目(31770640),黑龙江省留学归国基金项目(LC201512). （31770640）
