草业科学2018，Vol.35Issue(1)：76-84,9.DOI:10.11829/j.issn.1001-0629.2016-0190
化学法和生物法制备巨菌草腐植酸的比较
Comparison of chemical and biological methods to measure humic acid content in Pennisetum sp.
摘要
Abstract
The humic and fulvic acids in Pennisetum sp.were compared with chemical and biological methods. Cellulose,hemicellulose,and lignin were degraded to produce humic acid and fulvic acid with nitric acid,hy-drochloric acid,acetic acid,oxalic acid,and ammonia.The humic acid content was the highest when using 10.0% ammonia,followed by 37.5% nitric acid and 37.5% hydrochloric acid,while oxalic acid and acetic acid were the lowest.Bacillus subtilis,Mucor,Rhizopus,and Penicillium played a positive role in the formation of humic and fulvic acids.The degradation degree of lignin was the highest when using Rhizopus.The total hu-mic acid contents were 1.45 and 1.29 times that of 10.0% ammonia under Rhizopus and Mucor fermentation, respectively,while the total humic acid contents were less than 10.0% ammonia under both B.subtilis and Penicillium fermentation.The content of fulvic acid using 3 7.5% nitric acid was 1.7 9 times that of Mucor fer-mentation,and the content of fulvic acid using 22.5% hydrochloric acid was 1.98 times that of Mucor fermenta-tion.关键词
巨菌草/腐植酸/黄腐酸/纤维素/半纤维素/木质素/发酵Key words
Pennisetum sp./humic acid/fulvic acid/cellulose/hemicellulose/lignin/fermentation分类
农业科技引用本文复制引用
尚校兰,李宏宇,杨伊婷,梁菁菁..化学法和生物法制备巨菌草腐植酸的比较[J].草业科学,2018,35(1):76-84,9.基金项目
廊坊师范学院博士基金项目(LSLB201602) （LSLB201602）
河北省高校食药用菌应用技术研发中心(YF201411) （YF201411）
