重庆医学2018，Vol.47Issue(3)：299-301,305,4.DOI:10.3969/j.issn.1671-8348.2018.03.003
阿托伐他汀对K562细胞的抗增殖作用及凋亡作用的研究
Antiproliferative and apoptosis effect of atorvastatin on K562 cells
孔春芳 1周江龙 2丁伟荣 3丁江华 3陈国安 3程洪波 3金成豪3
作者信息
- 1. 江西省人民医院,南昌330006
- 2. 江西省南昌市第三医院 330006
- 3. 南昌大学第一附属医院 330006
- 折叠
摘要
Abstract
Objective To explore the effect of atorvastatin on the proliferation and apoptosis of K562 cells andto investigate its mechanisms.Methods The cells were treated by different concentrations of atorvastatin.The CCK-8 assay was employed to detect the cell proliferation.The cell apoptosis was detected by AnnexinV-FITC/PI dual staining;the flow cytometry was used to detect the cellular cycle;the activities of caspase-3,-8,-9 were detected by the colorimetric method;qRT-PCR was employed to measure the mRNA expression levels of Bcl-2 and PDCD5 in K562 cells.Results Atorvastatin could inhibit the proliferation of K562 cells in a time-and dose-dependent manner(P＜0.05);and induced the apoptosis of K562 cells,the percentage of G0/G1 phase cells was increased after atorvastatin treating k562 cells(P＜0.01),while the percentage of S phase cells was decreased(P＜0.01),moreover which showing the concentration dependence(P＜0.01);atorvastatin activated the caspase-3,-8,-9 (P＜0.01);down-regulated Bcl-2 mRNA expression and up-regulated PDCD5 mRNA expressionin a concentration dependence(P＜0.01).Conclusion Atorvastatin can inhibit the proliferation and induce apoptosis in K562 cells.关键词
细胞增殖/细胞凋亡/阿托伐他汀/K562细胞Key words
cell proliferation/apoptosis/atorvastatin/K562 cells分类
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孔春芳,周江龙,丁伟荣,丁江华,陈国安,程洪波,金成豪..阿托伐他汀对K562细胞的抗增殖作用及凋亡作用的研究[J].重庆医学,2018,47(3):299-301,305,4.
