重庆医学2018，Vol.47Issue(3)：340-345,6.DOI:10.3969/j.issn.1671-8348.2018.03.017
沉默MagT1影响大鼠心肌细胞内Mg2+水平和细胞凋亡的研究
Effect of silencing MagT1 on the content of magnesium ion and apoptosis in rat cardiomyocytes
摘要
Abstract
Objective The changes of Mg2+ concentration and cell apoptosis were detected by using siRNA to silence MagT1 in rat cardio myocytes.Methods MagT1 siRNA sequences were designed and synthetized,then transfected into primary cultured rat myocardial cell for silencing MagT1.The expression of MagT1 mRNA and protein were detected by RT-PCR and Western blot.The changes of Mg2+ concentration in the cells were detected by fluorescence microscopy.Flow cytometry (FCM) was used to detect the cell apoptosis.Results Compared with negative siRNA group,MagT1 siRNA transfected rat cardiomyocytes after 48 h,MagT1 mRNA silence efficiency was 51.83 ％ (P＜0.05),the silence of MagT1 protein efficiency was 56.75 ％ (P＜0.05),intracellular Mg2+ concentration was reduced by 29.13％ (P＜0.05),the apoptosis rate was 31.18％ (P＜0.01);MagT1 siRNA transfected rat cardiomyocytes after 60 h,MagT1 mRNA silence efficiency was 86.91％ (P＜0.01),the silence of MagT1 protein efficiency was 83.85％ (P＜0.01),intracellular Mg2+ concentration was reduced by 41.32％ (P＜0.01),the apoptosis rate was 40.61％ (P＜0.01).Conclusion After the silencing of MagT1,the concentration of Mg2+ in the cells decreased significantly,the apoptotic rate increased significantly,cell life activities are greatly affected.关键词
细胞凋亡/肌细胞,心脏/镁/镁离子转运蛋白1/siRNAKey words
cell apoptosis/myocytes, cardiac/magnesium/magnesium transporter 1/siRNA分类
医药卫生引用本文复制引用
滕飞翔,沈之君,赵洪侠,张栋梁,杨留才..沉默MagT1影响大鼠心肌细胞内Mg2+水平和细胞凋亡的研究[J].重庆医学,2018,47(3):340-345,6.基金项目
江苏省盐城市医学科技发展计划项目(YK2016046). （YK2016046）
