重庆医学2018，Vol.47Issue(3)：374-376,380,4.DOI:10.3969/j.issn.1671-8348.2018.03.027
贵阳地区2015年手足口病柯萨奇病毒A6、A10型的检测分析
Analysis of Coxsackie virus A6 and A10 detection in hand foot and mouth disease in Guiyang area during 2015
摘要
Abstract
Objective To understand the enterovirus(EV) pathogenic composition of Coxsackie virus(CV)A6 and A10 causing hand foot and mouth disesse(HFMD) in Guiyang area during 2015 to provide a basis for the prevention and treatment of HFMD in local area.Methods The specific primers were respectively designed according to CVA6 and CVA10 sequence published in GenBank.The fluorescence quantitative RT-PCR method system was established.The gene sequencing method was used for conducting verification.Then this method was used to detect the clinical samples from 607 cases of HFMD.Results A total of 607 samples of suspected HFMD were detected,the overall positive rate was 59.47 ％ (361/607),in which EV71 accounted for 7.25 ％ (44/607),CVA16 for 11.37％(69/607),EV71+CVA16 double positive accounted for 0.16％ (1/607) and other EV for 40.69％ (247/607).The positive samples of CVA6,CVA10 and CVA6+CVA10 detected by the established real time fluorescence RT-PCR were 11 cases,71 cases and 1 case.Conclusion CVA6 and CVA10 are the main pathogens causing new onset HFMD in Guiyang area and the CVA10 monitoring should be strengthened.关键词
手足口病/肠道病毒A型,人/基因型Key words
hand, foot and mouth disease/enterovirus A, human/genotype分类
医药卫生引用本文复制引用
杨兴林,洪章萍,王艺,李丽,梁跃东,黄海..贵阳地区2015年手足口病柯萨奇病毒A6、A10型的检测分析[J].重庆医学,2018,47(3):374-376,380,4.基金项目
贵州省贵阳市科技局项目(筑科合[2013103]9号) （筑科合[2013103]9号）
贵州省贵阳市高层次创新型青年卫生人才项目(筑卫合[2013]创2号). （筑卫合[2013]创2号）
