重庆医学2018，Vol.47Issue(4)：433-435,3.DOI:10.3969/j.issn.1671-8348.2018.04.001
腹泻型感染后IBS患者外周血和结肠黏膜IFN-γ、IL-10表达及临床意义
Expression of IFN-γ and IL-10 in peripheral blood and intestinal mucosa of patients with diarrhea post-infectious irritable bowel syndrome
摘要
Abstract
Objective To investigate the expression change of cytokines in peripheral blood and intestinal mucosa in the patients with diarrhea post-infectious irritable bowel syndrome(PI-IBS) and its relation with clinical symptoms scores.Methods Thirty outpatients and inpatients with diarrhea PI-IBS(observation group) and contemporaneous 30 individuals undergoing physical examination(control group) in the Hainan Provincial People's Hospital from January to December 2013 were selected.The peripheral blood mononuclear cells(PBMC) were separated and cultured.Then the levels of IFN-y and IL-10 in peripheral blood and cell culture supernatant fluid were detected by ELISA.The colonic mucosal tissue was taken by coloscopy.Then colonic mucosal IFN-γ and IL-10 protein expression was detected by immunohistochemistry staining.Furthermore,the correlationship between the level change of IFN-γ and IL-10 with clinical symptom score was analyzed by using the Spearman correlation method.Results Peripheral blod IL-10 and IFN-γ levels had no statistical difference between the two groups(P＞0.05).Compared with the control group,in PBMC seperation and cuture,the IFN-γ level in the observation group was increased and IL-10 level was decreased,the difference was statistically signifieant(P＜0.01).The intestinal main symptom score in the observation group had the positive correlation with IFN-γ expression level of PBMC culture supernatant fluid and colonic mucosal IFN-γ expression level(r=0.45,0.94,P＜0.01),and had the negative correlation with IL-10 expression level(r=-0.52,-0.79,P＜0.01).Conclusion The unbalance of IFN-γ and IL-10 level could be involved in the pathogenesis of diarrhea PI-IBS,which can serve as the observation indicators of disease activity.关键词
肠易激综合征/腹泻型/感染/干扰素Ⅱ型/白细胞介素10Key words
irritable bowel syndrome/diarrhea/infection/interferon type Ⅱ/interleukin-10分类
医药卫生引用本文复制引用
何周桃,孙晓宁,周旭春,黄白丽,陈益耀,邓桃枝,韩向阳,蓝程..腹泻型感染后IBS患者外周血和结肠黏膜IFN-γ、IL-10表达及临床意义[J].重庆医学,2018,47(4):433-435,3.基金项目
国家自然科学基金资助项目(81160057) （81160057）
海南省国际科技合作专项基金资助项目(KJHZ2013-14). （KJHZ2013-14）
