重庆医学2018，Vol.47Issue(4)：446-448,452,4.DOI:10.3969/j.issn.1671-8348.2018.04.004
EGF对人毛囊外毛根鞘无色素性黑素细胞增殖及迁移能力的影响
Effect of epidermal growth factor on proliferation and migration ability of amelanotic melanocytes in human hair follicle outer root sheath
袁睿 1杜宇2
作者信息
- 1. 重庆市渝北区人民医院皮肤科 401100
- 2. 西南医科大学附属医院皮肤科,四川泸州646600
- 折叠
摘要
Abstract
Objective To investigate the effect of epidermal growth factor (EGF) on proliferation and migration ability of amelanotic melanocytes (AMMC) in human hair follicle outer root sheath.Methods AMMC were purified to the third generation by the variant enzyme method.Then AMMC was identified by the immunohistochemical method;the changes of cellular proliferation and migration ability after intervention by different concentrations of EGF were detected by using CCK8 and Tianswell in vitro chamber migration test.Results The third generation AMMC immaro histochemical staining showed NKI/beteb staining positive and HMB-45 staining negative.The OD values of CCK8 test and the Transwell in vitro chamber migration assay results showed that its promoting proliferation and migration effects were increased with the EGF concentration increase,but when further increasing concentration,the effects had no obvious enhancement.Conclusion A certain concentration range of EGF in vitro can promote AM-MC proliferation and migration.关键词
毛囊外毛根鞘/无色素性黑素细胞/细胞增殖/细胞运动/表皮生长因子Key words
out root sheath/amelanotic melanocytes/cell proliferation/cell movement/epidermal growth factor分类
医药卫生引用本文复制引用
袁睿,杜宇..EGF对人毛囊外毛根鞘无色素性黑素细胞增殖及迁移能力的影响[J].重庆医学,2018,47(4):446-448,452,4.
