中国妇幼健康研究2018，Vol.29Issue(1)：45-48,4.DOI:10.3969/j.issn.1673-5293.2018.01.011
植入前遗传学筛查中不同形态特征的胚胎整倍体率比较
Comparison of euploid rates among embryos with different morphological features in preimplantation genetic screening
摘要
Abstract
Objective To compare the euploidy and aneuploidy rates of embryos with different morphological parameters by analyzing the chromosome results and embryos of patients in preimplantation genetic screening(PGS).Methods The patients who underwent preimplantation genetic screening in the Center for Assisted Reproduction of Northwest Women and Children's Hospital during January 1,2015 to April 30,2017 were collected.Their embryos were cultured to biopsy in the cleavage and blastocyst stage. Array-CGH(BlueGnome 24SureV3)was adopted for PGS.The main parameters for the evaluation of cleavage stage were the number of blastomeres on D3,the ratio of fraction volume and the number of non-uniform blastomeres.The blastocyst was evaluated by Garnder blastocyst grading.The morphological parameters of the embryos and the incidence of euploidy were compared.Results A total of 348 embryos were biopsied by PGS and 322 embryos were successfully detected.The number of euploid embryos was 123(38.2%)while the number of aneuploid embryos was 199(61.80%).The difference in the ratio of fraction volume during cleavage stage between euploid group and aneuploid group was statistically significant(t= -2.60,P<0.05).There were no statistically significant differences in the number of blastomeres and the number of non-uniform blastomeres on D3.There was statistically significant difference in the incidence of euploidy and aneuploidy in the expansion stage of blastocyst during biopsy(χ2= 2.00,P<0.05).However,there were no statistically significant differences in biopsy time(D4-D5,D6), morphology of inner cell mass(A/B,C)and ectoderm morphology(A/B,C)(χ2value was 0,0 and 1.69,respectively,all P<0.05).Conclusion By analyzing the chromosome results of patients in preimplantation genetic screening,in the cleavage stage embryo evaluation parameters,the embryos with low ratio of fragments volumes are of higher incidence of euploidy,while in blastocyst morphological parameters,the embryos with higher expansion stage of blastocyst during biopsy are of higher incidence of euploidy.关键词
植入前遗传学筛查/整倍体/非整倍体/胚胎碎片/囊胚形态Key words
preimplantation genetic screening(PGS)/euploidy/aneuploidy/embryonic fragments/blastocyst morphology分类
医药卫生引用本文复制引用
施文浩,张四林,王永博,师娟子..植入前遗传学筛查中不同形态特征的胚胎整倍体率比较[J].中国妇幼健康研究,2018,29(1):45-48,4.基金项目
中华医学会临床医学科研专项资金———生殖医学青年医师研究与发展专项资金资助项目(编号:16020240640) （编号:16020240640）
