高校地质学报2018，Vol.24Issue(1)：33-40,8.DOI:10.16108/j.issn1006-7493.2017023
振荡与静置条件下细菌诱导形成的碳酸钙矿物类型与形态的对比
Species and Morphologies of Calcium Carbonates Induced by Bacteria under Shake and Static Culture
摘要
Abstract
To better explore the effect of culture method(static and shake cultures)on species and morphologies of carbonate minerals induced by bacteria,a batch of oscillatory experiments were carried out for a duration of 40 days in B4 medium under the mediation of three kinds of bacteria-Bacillus cereus(strain GW-1), Lysinibacillus sp.(strain GW-2) and Microbacterium sp.(strain GW-3). At the same time,aseptic experiment without the inoculation was done as the control.The mineral species of carbonate were determined by X-ray diffraction(XRD), and morphologies of precipitated carbonates were observed using scanning electron microscopy(SEM). The results obtained under oscillatory incubation condition were compared with those obtained under static condition. The main results were as follows:(1) All three bacterial strains could induce precipitate of calcium carbonate, but they had different abilities. Their mineralization abilities were in the order of GW-2>GW-1>GW-3;(2) At the two culture methods, strains GW-1 and GW-2 induced the formation of calcite, while strain GW-3 mediated the formation of vaterite. The results suggested that bacterial species were the important factors affecting mineral species. Culture methods almost had no effect on mineral species;(3) The morphologies of minerals induced by strains GW-1 and GW-2 were diversified with shake culture. However, single morphology existed in the samples with static culture. The morphology of all minerals formed by strain GW-3 was spherical shape and was not related to the culture methods used.关键词
方解石/球霰石/形态/细菌/振荡培养/静置培养Key words
calcite/vaterite/morphology/bacteria/shake culture/static culture分类
天文与地球科学引用本文复制引用
郭文文,李福春,张宠宏..振荡与静置条件下细菌诱导形成的碳酸钙矿物类型与形态的对比[J].高校地质学报,2018,24(1):33-40,8.基金项目
国家自然科学基金项目(41673083 （）
41172308) （）
中国科学院黄土与第四纪地质国家重点实验室开放课题(SKLLQG1309) （SKLLQG1309）
山东省自然科学基金项目(ZR2016DB34) （ZR2016DB34）
枣庄学院博士科研启动基金项目(2013BS03)联合资助 （2013BS03）
