高校化学工程学报2018，Vol.32Issue(1)：138-146,9.DOI:10.3969/j.issn.1003-9015.2018.01.019
pgi基因敲除对大肠杆菌利用混合碳源的影响
Effects of pgi Knockout on Mixed Carbon Source Utilization of Escherichia Coli
摘要
Abstract
Lignocellulosic biomass is an attractive carbon source for bio-based fuel and chemical production. However, carbon catabolite repression and inability to use multiple sugars derived from lignocellulosic biomass hinder its commercial usage. This research constructed an Escherichia coli △pgi strain, which can simultaneously consume glucose, fructose and xylose. The effects of modulating pgi (△pgi) gene on the consumption of multiple carbon sources were investigated under both aerobic and anaerobic conditions. The results show that three carbon sources are consumed simultaneously with pgi knockout.The overall regulation mechanisms are clarified based on fermentation data, gene transcript analysis and flux analysis. The knockout of pgi gene leads to the degradation of ptsG transcription, increase of cAMP-Crp and relaxation of catabolite repression, which may affect co-consumption of multiple carbon sources.关键词
大肠杆菌/pgi敲除/碳源共利用/代谢调控Key words
Escherichia coli/pgi knockout/simultaneous carbon source utilization/catabolite regulation分类
化学化工引用本文复制引用
潘柯莉,茅以诚,岳圣杰,姚瑞莲,胡洪波..pgi基因敲除对大肠杆菌利用混合碳源的影响[J].高校化学工程学报,2018,32(1):138-146,9.基金项目
国家自然科学青年基金(31400086,81402956) （31400086,81402956）
中国博士后科学基金(2016M601584) （2016M601584）
上海市科委晨光计划(14CG03). （14CG03）
