高校化学工程学报2018，Vol.32Issue(1)：193-199,7.DOI:10.3969/j.issn.1003-9015.2017.00.049
光响应茶皂苷元纳米囊的制备及其抗耐药菌活性研究
Preparation of Photo Responsive Sapogenin Nanocapsules and their Inhibiting Activity on Drug Resistant Bacteria
摘要
Abstract
Photo responsive sapogenin nanocapsules were prepared by a dual emulsification method to improve their stability and inhibiting activity on drug resistant bacteria. When soy lecithin, cholesterol and phaeophorbide are used as nanocapsule materials with a mass ratio of 50:10:1, the encapsulation efficiency, drug loading capacity, average particle size and zeta potential are 87.3%±4.6%, 13.5±1.2%, (155±5) nm and(-43.3±2.0) mV, respectively. The nanocapsule has a slow release effect without irradiation, but its drug release rate accelerates after irradiation at 650 nm. Its 50% inhibitory concentrations on drug resistant Escherichia coli and Staphylococcus aureus are 52.5 and 85.4 μg?mL-1,respectively,which decrease to 22.6 and 45.7 μg?mL-1respectively when combined with gentamycin (10 μg?mL-1). This result indicates that the nanocapsule can reduce and reverse bacterial resistance against gentamycin. Molecular docking results show that there are remarkable interactions between sapogenin and mannitol dehydrogenase (MDH) or Penicillin binding proteins (PBPs), which mean that the nanocapsules are involved in the inhibition of drug resistant bacteria.关键词
茶皂苷元/纳米囊/光响应/分子对接/抗菌活性/耐药性Key words
sapogenin/nanocapsule/photo response/molecular docking/antibacterial effect/drug resistance分类
化学化工引用本文复制引用
李穗敏,杨谦,赵闯,赵俊,叶勇..光响应茶皂苷元纳米囊的制备及其抗耐药菌活性研究[J].高校化学工程学报,2018,32(1):193-199,7.基金项目
广州市科技计划项目(1563000123) （1563000123）
广东省部产学研项目(2016B090918086). （2016B090918086）
