广西植物2018，Vol.38Issue(1)：65-75,11.DOI:10.11931/guihaia.gxzw201701023
基于DGGE研究喀斯特金橘接种AMF对根际细菌多样性和植株生长的影响
Effects of Fortunella margarita inoculated with AMF on rhizospheric bacterial community diversity based on DGGE
摘要
Abstract
To overcome the soil infertility and poor crop output problem of karst region,we chose Funneliformis mosseae (F.m)and Claroideoglomus etunicatum(C.e)as AMF inoculants and two types of soil in karst region as potted matrix, the bacterial community diversity and growth of Fortunella margarita were investigated by PCR?DGGE(denaturing gra?dient gel electrophoresis)technique and other conventional approaches. The results were as follows: Comparing to no AM fungi treatment,the colonization rate of root system of F. margarita such as F,m,v inoculated with either C.e or F.m treatment increase significantly. No matter in either type of soil,C.e treatment are the highest. In addition,rhizos?pheric soil enzyme activities including invertase,phosphatase,protease and urease with either C.e or F.m treatment in?crease significantly. Among them,activities of protease and urease are the highest in lime soil(LS),and that of invert?ase and phosphatase in acid soil(AS)are higher than that in LS, the differences are significant with each other (P<0.05). Either in AS or LS,the four kinds of soil enzyme activities with C.e are the highest.In terms of bacterial di?versity,the bands number of bacterial DNA with AMF of DGGE profile enriched,and the index of richness(R),diver?sity(H)and evenness(E)with AMF are higher than no AMF treatment.No matter in either type of soil,value of R,H and E with C.e are the highest.Additionally,based on the results of sequence analysis to DNA dominant bands of DGGE profile,all dominant bacterial strains are phylogenetically closen to uncultured microbes,including Acidobacterium,Pro?teus,Rhizobium and Actinomyce,the similarity of each is above 97%. Moreover,comparing to no AM fungi treatment, biomass of Fortunella margarita inoculated with either C.e or F.m treatment increases significantly,C.e treatment are the highest in AS. In conclusion,comparing to F.m,C.e represents better efficiency for F. margarita in karst region. AMF can enhance activities of four rhizospheric soil enzyme,stimulated diversities of soil bacteria population. As the result, plant biomass increase.It provides the information for improving growth of F.margarita by facilitating the conversion and dynamics of nutrients such as C,N,P and others.关键词
喀斯特地区/金橘/丛枝菌根真菌/土壤酶/PCR⁃DGGE/细菌群落结构Key words
karst region/Fortunella margarita/arbuscular mycorrhizal fungi(AMF)/soil enzyme/PCR⁃DGEE/bacterial community structure分类
农业科技引用本文复制引用
雷芸,林钰,胡振兴,邓荫伟,田远春,刘灵..基于DGGE研究喀斯特金橘接种AMF对根际细菌多样性和植株生长的影响[J].广西植物,2018,38(1):65-75,11.基金项目
广西自然科学基金(2013GXNSFAA019094) （2013GXNSFAA019094）
广西高校科学技术研究重点项目(ZD2014015) （ZD2014015）
桂林市科学研究与技术开发项目(20120119?4)[Supported by the Natural Science Foundation of Guangxi(2013GXNSFAA019094) （20120119?4）
Key Program of Science and Technology of Guangxi Universities(ZD2014015) （ZD2014015）
Scientific Research and Technical Development Plan Program of Guilin(20120119?4)]. （20120119?4）
