广西植物2018，Vol.38Issue(1)：119-127,9.DOI:10.11931/guihaia.gxzw201702017
抑制水稻细菌性条斑病菌的没食子酸分离及其对水稻细菌性条斑病的防治作用
Gallic acid isolated from Sedum lineare and its control efficacy on rice bacterial leaf streak
摘要
Abstract
A compound,which could significantly inhibit the growth of Xanthomonas oryzae pv.oryzicola(Xoc)in vitro, was isolated from Sedum lineare by liquid?liquid extraction,silica gel column chromatography and gel column chromatog?raphy. Based on the spectral data,including 1H and 13C NMR data,this compound was identified as 3,4,5?Trihydroxy?benzoic acid,namely gallic acid(GA). The results of the test,on determining the antibiotic spectrum of GA at concen?tration 30 mg·mL?1, indicated that GA could most strongly inhibit the growth of X.campestris pv. pruni, X. oryzae pv. oryzicola,X. oryzae pv. oryzae and X. axonopodis pv. citri,with inhibition zones width of 25.33,24.00,22.33 and 20.67 mm, respectively, and had fair anti?bacteria activity against Ralstonia Solanacearum, Pseudomonas syringae pv. glycinea, P. syringae pv. tomato and Pectobacterium carotovora subsp. carotovora, with inhibition zones width of 18.00,12.33,11.00 and 8.68 mm,respectively. Meanwhile,GA could weakly inhibit the growth of eleven pathogenic fungi,including Phytophthora nicotianae, Penicillium digitatum, Streptobotrys streptothrix, Pythium aphanidermatum, Pestalotiopsis mangiferae, Curvularia lunata, Rhizoctonia solani, Fusarium oxysporum f. sp. niverum, F. oxysporum f. sp. nicotianae, Botrytis cinerea and Sclerotium rolfsii, with inhibition ratio of 56.34%, 54.73%, 38.00%, 29.86, 28.17%,20.00%,19.02%,16.71%,10.59%,4.58% and 1.96%, respectively. Growth inhibition exceeding 50% by GA was only observed in Phytophthora nicotianae and Penicillium digitatum. The growth of Botrytis cinerea and Scleroti?um rolfsii was slightly inhibited by GA.In potted experiment,gallic acid at 200,300 and 400 mg·L?1provided 63.55%, 71.38% and 77.39% control efficacy to rice bacterial leaf streak,respectively. At concentration of 300 mg·mL?1, GA could control rice bacterial leaf streak,with control efficacy of 64.62%in field.The study reveals that the gallic acid has the potential to be further developed as a bactericide to control rice bacterial leaf streak.关键词
抑菌物质/提取和纯化/佛甲草/没食子酸/水稻细菌性条斑病/防治效果Key words
anti⁃bacterial substance/extraction and purification/Sedum lineare/gallic acid/rice bacterial leaf streak/control efficacy分类
农业科技引用本文复制引用
汪锴豪,魏昌英,谢慧婷,袁高庆,林纬,范腕腕,黎起秦..抑制水稻细菌性条斑病菌的没食子酸分离及其对水稻细菌性条斑病的防治作用[J].广西植物,2018,38(1):119-127,9.基金项目
广西自然科学基金(2014GXNSFAA118073)[Supported by Guangxi Natural Science Foundation of China(2014GXNSFAA118073)]. （2014GXNSFAA118073）
