家畜生态学报2018，Vol.39Issue(2)：12-17,6.
let-7b介导IGF2BP3表达抑制鸡成肌细胞增殖效果的研究
MiRNA let-7b Inhibits Cell Proliferation in Chicken Myoblasts by Targeting to IGF2BP3
摘要
Abstract
In order to investigate the role of miRNA let-7b mediated by IGF2BP3 in the proliferation of chicken myoblasts,the let-7b overexpression vector was transfected into myoblasts and verified by MTT,qPCR,western blotting and ELISA,the expression of related genes was determined.The results showed that:(1) The double-luciferase reporter system and the mutant experiments demonstrate that let-7b acts on the 3'UTR of IGF2BP3.(2) Let-7b overexpression in myoblasts,cell proliferation was affected to a certain extent,compared with the empty plasmid group,the number of cells showed a downward trend.(3) Let-7b reduced the mRNA expression of target gene IGF2BP3 by 2.33 times,and the difference was significant (P＜0.05),and there was no significant difference in the mRNA expression of IGF2 and IGF1 (P＞0.05).(4) Comprehensive analysis,miRNA let-7b is mainly through the regulation of IGF2BP3 mRNA and protein expression levels,and further affect the transport,stability and migration of IGF2 function,thus affecting the proliferation of chicken myoblasts.关键词
鸡/miRNA/let-7b/成肌细胞/IGF2BP3Key words
chicken/miRNA/let-7b/myoblasts/IGF2BP3分类
农业科技引用本文复制引用
岳孝亭,刘燊,杨承忠,吕敏芝,郭金彪,张绮琼,刘洁珠,林树茂..let-7b介导IGF2BP3表达抑制鸡成肌细胞增殖效果的研究[J].家畜生态学报,2018,39(2):12-17,6.基金项目
广东省自然科学基金(2016A030313377) （2016A030313377）
