家畜生态学报2018，Vol.39Issue(2)：18-23,6.
少孢节丛孢菌XJ-A1几丁质酶基因AO-587和AO-31的克隆及分子特征分析
Cloning and Bioinformatics Analysis of the Chitinase Gene AO-587 and AO-31 of Arthrobotrys oligospora XJ-A1
摘要
Abstract
In order to study the function of chitinase in Arthrobotrys oligospora in Xinjiang,the XJ-A1 chitinase AO-587 and AO-31 genes were amplified,cloned and sequencing.After sequencing,the gene and its encoded protein were taken to do bioinformatics and molecular feature analysis.The results showed that both chitinases belonged to the family of glycoside hydrolase 18 and contained the conserved sequence SXGG and VDGXDLDL in the chitinase catalytic region.AO-587 contains a signal peptide sequence,indicating that this protein belongs to the secretory protein.These two chitinase tertiary structures are similar,with a (α/β) 8 cylindrical structure.Phylogenetic analysis showed that the chitinase from different sources can be divided into three different evolutionary branches according to the difference of molecular weight,among which the isolation of chitinase AO-587 and AO-31 were closed with that of Drechslerells stenobrocha isolates,indicating that they may play similar roles in the process of infecting the host-nematode.关键词
少孢节丛孢菌/几丁质酶/基因克隆/生物信息学Key words
Arthrobotrys oligospora/chitinase/gene cloning/bioinformatics分类
农业科技引用本文复制引用
钟文强,孟庆玲,乔军,陈英,贡莎莎,王熙凤,才学鹏..少孢节丛孢菌XJ-A1几丁质酶基因AO-587和AO-31的克隆及分子特征分析[J].家畜生态学报,2018,39(2):18-23,6.基金项目
国家自然科学基金(31460654,31260601) （31460654,31260601）
新疆自治区研究生科研创新项目(XJGRI2015038) （XJGRI2015038）
