家畜生态学报2018，Vol.39Issue(2)：39-44,6.
柠檬酸对小鼠卵泡组织形态的影响
Effects of Citric Acid on Histomorphology Changes of Developmental Follicular in Mice
摘要
Abstract
This research was conducted to investigate the effects of citric acid on various stages of development and morphology for follicles on female mice.60 Kunming female mice weighing 20-22 g were randomly divided into four groups including control group and low-dose,middle-dose,high-dose Citric acid (CA)-treated groups.Experiment group mice were respectively injected intraperitoneally with 0.2 ml CA ranging from 7.5,15 to 30 mg/ml once daily,and the same amount of physiological saline to control group.10 mice were randomly chosen from each group and were sacrificed at 21 d after injected decapitation under light ether anesthesia.The ovaries immersed in 4％ formaldehyde solution for 24 h,then was made into paraffin tissue section with HE stained and optical microscope observation.The results show that the morphology and quantity of follicles at different levels were normal in control group,the normal form of primitive follicles and primary follicles were observed for 7.5 mg/ml or 15 mg/ml citric acid group.Because of primordial follicle and primary follicles degraded,the number of primordial follicle or primary follicle significantly reduced in 30 mg/ml citric acid group,there were atresia on secondary follicle and antral follicle.It is concluded that low-dose and middle-dose citric acid don't affect development of original follicle and primary follicle,secondary follicle development were blocked by middle-dose citric acid and antral follicle cellular structure degradation.High-dose citric acid can significantly impact development of various follicle and cause follicular atresia.关键词
柠檬酸/小鼠/卵泡/颗粒细胞/卵泡膜Key words
citric acid/mice/follicles/granulosa cells/theca cells分类
农业科技引用本文复制引用
邓雯,吕琼霞,刘玉梅,陈晓光,马彦博..柠檬酸对小鼠卵泡组织形态的影响[J].家畜生态学报,2018,39(2):39-44,6.基金项目
河南科技大学科研创新能力培育基金(ZCX022) （ZCX022）
