扬州大学学报（农业与生命科学版）2017，Vol.38Issue(4)：1-8,8.DOI:10.16872/j.cnki.1671-4652.2017.04.001
不同耐热性新城疫病毒株全基因组比较研究
Comparative study on complete genomes of different heat resistant Newcastle disease virus strains
摘要
Abstract
The study on molecular biology of Newcastle disease virus (NDV) heat resistant (HR) strains is of great significance to the prevention and control of Newcastle disease.The complete genome sequences of all NDV HR strains and thermolabile strain LaSota were retrieved from GenBank.The compositions of nucleic acid of genome and amino acid of structural protein and the secondary structure and functional sites of capsule membrane protein were compared by bioin-formatic methods.The GC content of complete genome of the HR strains was slightly higher than that of the thermolabile strain LaSota,and the GC distribution in the genome was unbalanced,and there was a higher GC content area near the 3'end of the genome.The composition of polar amino acids,heterocyclic amino acids and proline in the amino acid sequences of HR strain structure protein was higher.The theoretical pI and instability index of F protien of HR strains was lower than thermolabile strain LaSota.F protein secondary structure of the spiral area of HR strains was expanded,but the folding area was smaller.The theoretical pI of HN protein of HR strains was lower than thermolabile strain LaSo ta.But the instability index of HN protein of HR strains was higher than thermolabile strain LaSota.Both spiral and folding areas of HN protein were expanded and it had one more alpha helix than the thermolabile strain LaSota.关键词
新城疫病毒/耐热株/全基因组/生物信息学/比较Key words
Newcastle disease virus/heat-resistant strains/complete genomes/bioinformatics/comparison分类
农业科技引用本文复制引用
曹永忠,阮宝阳,张小荣,胡海,陈银,吴艳涛..不同耐热性新城疫病毒株全基因组比较研究[J].扬州大学学报（农业与生命科学版）,2017,38(4):1-8,8.基金项目
国家重点研发计划项目(2016YFD0501604) （2016YFD0501604）
江苏省自然科学基金资助项目(BK20141274) （BK20141274）
扬州大学创新培育基金项目(2013CXJ063) （2013CXJ063）
