扬州大学学报（农业与生命科学版）2017，Vol.38Issue(4)：9-13,24,6.DOI:10.16872/j.cnki.1671-4652.2017.04.002
表面展示RGD模体重组噬菌体的构建和鉴定
Construction and identification of a recombinant phage vector display RGD motif for gene delivery
摘要
Abstract
The coding sequence of RGD peptide was cloned to PⅢ gene of helper phage M13KO7,obtaining recombinant vector M13KO7 RGD.Recombinant phage particles were prepared after packaging pAAV-GFP phagemid by M13KO7-RGD.Morphologies was observed by transmission electron microscopy,and expression of PⅢ RGD was identified by Western-blot.Phage particles were transduced to different mammalian cells and expression of reporter gene was observed by fluorescence microscope.Results showed that recombinant phage particles displayed RGD motif were obtained successfully,which had typical characteristics of filamentous phage.Green fluorescent protein was expressed after transduction of recombinant phage particles to different animal cells.These results indicated that the recombinant phage can be successfully transformed into mammalian cells,which provides a new idea for research and development of animal genetic engineering vaccine vectors.关键词
重组噬菌体/RGD模体/基因导入Key words
recombinant phage/RGD motif/gene delivery分类
生物科学引用本文复制引用
夏文龙,余树培,郭长明,朱善元,张鑫宇,夏晓莉,孙怀昌..表面展示RGD模体重组噬菌体的构建和鉴定[J].扬州大学学报（农业与生命科学版）,2017,38(4):9-13,24,6.基金项目
江苏省自然科学基金资助项目(BK20151576) （BK20151576）
江苏高校优势学科建设工程项目(PAPD) （PAPD）
江苏省农业科技自主创新资金项目[CX(14)2087] （14）
江苏农牧科技职业学院科研项目(NSFPT201631) （NSFPT201631）
