扬州大学学报（农业与生命科学版）2017，Vol.38Issue(4)：14-17,4.DOI:10.16872/j.cnki.1671-4652.2017.04.003
禽源大肠埃希菌ETT2毒力岛Duplex PCR检测
Duplex PCR detection of ETT2 pathogenicity island of avian Escherichia coli
摘要
Abstract
The virulence genes of avian pathogenic Escherichia coli (APEC) are more complex,especially the pathogenicity island ETT2 is rarely reported in recent research.In this study,the ECs3703 and ECs3737 genes were used as the markers for the detection of the ETT2 element,and a Duplex PCR method was established to rapid detect the ETT2-harboring avian E.coli.The specificity of the PCR was confirmed by using reference strains.187 samples from diseased poultry were submitted to Duplex PCR detection,and the results revealed that 77 (41.2％) were infected with ETT2-harboring avian E.coli.A total of 247 bacteria isolates were made further identified by the PCR method,and the data showed that 116 (46.9％) isolates were positive for ETT2 pathogenicity island.关键词
禽/大肠埃希菌/ETT2毒力岛/PCR检测Key words
avian/Escherichia coli/ETT2 pathogenicity island/PCR detection分类
农业科技引用本文复制引用
黄丹,成大荣,宋晓莉,宋海港,石文弢..禽源大肠埃希菌ETT2毒力岛Duplex PCR检测[J].扬州大学学报（农业与生命科学版）,2017,38(4):14-17,4.基金项目
江苏现代农业(水禽)产业技术体系集成创新中心项目(SXGC[2017]248) （水禽）
江苏省六大人才高峰项目(2014-NY-023) （2014-NY-023）
