扬州大学学报（农业与生命科学版）2017，Vol.38Issue(4)：18-24,7.DOI:10.16872/j.cnki.1671-4652.2017.04.004
广西巴马地区人群肠道菌群的影响因素研究
Study on the influencing factors of fecal microflora in people living in Bama, Guangxi, China
摘要
Abstract
Bama county (Guangxi,China) is the world longevity village,in this study,intestinal gut microflora of different population in Bama county was analyzed,and the effect of age,village and gender on the fecal microflora in longevity village was discussed.The number of Enterobacter spp.,Enterococcus spp.,Clostridium leptum,Lactobacillus spp.,Bacteroides spp.,and Bifidobacterium spp.in 28 healthy subjects feces from 3 village was quantified by real-time PCR.The results showed that the number of Bifidobacterium spp.in 28 healthy subjects feces in Bama county was higher than other five flora,and the Enterococcus spp.was the lowest.Significant differences were observed in fecal microflora in different age groups,the number of Lactobacillus in longevous (mean age=96 years;n=6) was significantly higher than middle-age (mean age-43 years;n=5) and elderly (mean age=79 years;n=9) (P＜0.05),the number of Bifidobacterium spp.in youth (mean age=12 years;n =8) and longevous was significantly higher than middle-age and elderly (P＜0.05),and the number of Enterobacter spp.in youth was significantly lower than middle-age (P＜0.05);the number of Bacteroides spp.in elderly was significantly lower than longevous (P＜0.05),the number of Enterococcus spp.in youth and longevous was significantly lower than middle-age and elderly (P＜0.05),the number of C.leptum in elderly and longevous was significantly lower than youth and middle-age (P＜0.05);Significant differences were not observed in the six flora in different village and gender population feces.This study suggests that age can affect the fecal microflora of people in the longevity county of Bama.Age had more influence on the intestinal flora than gender and village in the Bama longevity area.关键词
人群/长寿之乡/不同年龄/粪便菌群/荧光定量PCRKey words
people/longevity village/age/fecal microflora/real-time PCR分类
医药卫生引用本文复制引用
陈大卫,范晨雨,鲁茂林,郭飞翔,顾瑞霞,伍云,李正华..广西巴马地区人群肠道菌群的影响因素研究[J].扬州大学学报（农业与生命科学版）,2017,38(4):18-24,7.基金项目
国家自然科学基金青年基金项目(31701627) （31701627）
国家自然科学基金面上项目(31571855) （31571855）
江苏省高校自然科学研究面上项目(17KJB550009) （17KJB550009）
江苏省高校自然科学研究重大项目(17KJA550004) （17KJA550004）
江苏省乳品生物技术与安全控制重点实验室开放课题(K14003) （K14003）
