扬州大学学报（农业与生命科学版）2017，Vol.38Issue(4)：39-45,7.DOI:10.16872/j.cnki.1671-4652.2017.04.008
13个地方鸡品种6个微卫星座位遗传多样性分析
Analysis of genetic diversity of thirteen indigenous chicken breeds using six microsatellite loci
摘要
Abstract
In this paper,six microsatellite markers with high polymorphisms were selected to detect the genetic diversity of thirteen chicken breeds.The alleles frequencies,polymorphism information content (PIC) and average heterozygosity within each population,DA and Ds genetic distance among breeds were analyzed.The application of microsatellite polymorphisms to the detection of genetic variability and relationship among populations was discussed.Altogether,eightythree alleles were found in this experiment,the polymorphic information content was highly polymorphic and it can be used as an effective genetic marker for genetic diversity and phylogenetic relationships in chicken breeds.Heterozygosity of thirteen chicken breeds were high,which was between 0.746 4 and 0.870 0.The genetic distances of DA and Ds in 13 chicken breeds were the same,but DA was larger than Ds.UPGMA tree was completed through analysis of DA and Ds genetic distance.In the tree,the Dongxiang Green Shell chicken,Chahua chicken,Shouguang chicken,Huaixiang chicken,Huainan Pockmarked chicken,Bian chicken,Wenchang chicken,Luyan chicken,Beijing Fatty chicken and Gushi chicken were grouped together.The Xuhai chicken,Zang chicken and Youxi Pockmarked chicken were grouped together.In summary,the UPGMA tree well reflected the evolutionary and breeding history of the thirteen breeds.关键词
地方鸡/微卫星/遗传多样性Key words
indigenous chicken breeds/microsatellite/genetic diversity分类
农业科技引用本文复制引用
赵东伟,李春苗,黎寿丰,卢建,赵振华,黄华云,王钱保,黄正洋..13个地方鸡品种6个微卫星座位遗传多样性分析[J].扬州大学学报（农业与生命科学版）,2017,38(4):39-45,7.基金项目
国家现代农业产业技术体系建设专项(CARS-42-Z06) （CARS-42-Z06）
江苏省农业三新工程项目[SXGC(2016)206] （2016）
江苏省科技支撑计划项目(BE2015344) （BE2015344）
江苏省农业科技自主创新基金项目[CX(15)1009] （15）
扬州市自然科学基金资助项目(YZ2015104) （YZ2015104）
