扬州大学学报（农业与生命科学版）2017，Vol.38Issue(4)：94-98,104,6.DOI:10.16872/j.cnki.1671-4652.2017.04.017
瓜蚜取食对黄瓜几种保护酶活性的影响
Effect of Aphis gossypii Sucking on three protective enzymes activity of cucumber
摘要
Abstract
In order to investigate the effects of melon aphid feeding on several protective enzymes,peroxidase isozyme (POD),polyphenol oxidase (PPO) and phenyalanine ammonialyase (PAL) activity in leaves of 2 cucumber c ultivars,middle resistant cultivar Zao2 N and susceptible cultivar Sichuan Cunjin,were measured after different density (50,100,200 respectively) of melon aphid feeding at different time (24,48,72,96 h after feeding).The results showed that the activities of POD,PPO and PAL all increased rapidly,and these in resistant cultivar were all higher than in susceptible cultivar.The activities of POD and PAL in two cultivars were increased to peak value at 96 and 48 h respectively.The PPO activity in resistant cultivar reached to peak value at 72 h while at 48 h in susceptible cultivar.Different density of insects feeding in our experiment made no significant difference in enzyme activity of leaves.From our results,we can conelude that there was an accumulation and enhancing effects on the induction of three enzymes in the leaves of cucumber,and the threshold of the induced intensity was found.We could use these 3 kinds of enzyme activity in cucumber leaves as aphid resistant index.The results provide a theoretical basis for the further application of cucumber insect resistance to the ecological control of aphids.关键词
黄瓜/瓜蚜/过氧化物酶/多酚氧化酶/苯丙氨酸解氨酶/诱导抗虫性Key words
cucumber/Aphis gossypii Glover/peroxidase isozyme/polyhenol oxidase/phenyalanine ammonialyase/aphid-resistance分类
农业科技引用本文复制引用
高红胜,任佳,吴晓霞,张海波,陈学好,周福才..瓜蚜取食对黄瓜几种保护酶活性的影响[J].扬州大学学报（农业与生命科学版）,2017,38(4):94-98,104,6.基金项目
江苏省科技支撑计划项目(BE2017347) （BE2017347）
江苏省农业科技自主创新资金项目[CX(17) 2004] （17）
江苏省农业三新工程项目[SXGC(2017)303] （2017）
苏州市产业技术创新专项(农业科技创新工程)(SNG201504) （农业科技创新工程）
