检验医学与临床2018，Vol.15Issue(4)：439-443,5.DOI:10.3969/j.issn.1672-9455.2018.04.003
HBV感染患者氧化应激状态与病毒基因型和耐药突变的相关性
Relationship between oxidative stress in patients with HBV-induced liver disease and HBV genotype/drug-resistant mutation
摘要
Abstract
Objective To explore the correlation of oxidative stress in patients with chronic hepatitis B and degree of severity of the disease with HBV genotype and drug-resistant mutation.Methods A total of 296 pa-tients with diagnosed chronic hepatitis B were selected from Feb.2014 to Apr.2016 in Mianyang Central Hos-pital,included 145 patients with chronic hepatitis B(CHB),101 patients with hepatitis B cirrhosis(HBC)and 50 patients with CHB-related hepatocellular carcinoma(HCC).Three HBV genotypes(B,C and D)and eight drug-resistant mutation genes(rt180L,rt204M,rt207V,rt236N,rt250M,rt181A,rt184T,rt202S) were detec-ted by PCR-reverse dot blot method.In addition,total oxidative stress(TOS),and total antioxidant status (TAS)were measured,on the basis of which oxidative stress index(OSI)was calculated.Furthermore,then the differences of TOS,TAS and OSI levels were compared between different liver diseases,different geno-types or drug-resistant mutation,and also the correlations were analyzed between HBV genotype,drug-resist-ant mutation,patient′s oxidative stress status and disease severity.Results Serum TOS and OSI levels,HBV-C ratios and drug-resistant mutation rates were increased gradually with the severity of liver disease(CHB<HBC< HCC,P<0.05).Serum TAS levels decreased with degree of severity of the disease,but there was no statistical difference between CHB group and HCC group(P>0.05).Except TAS levels in patients at HBC group,compared with patients without mutation in HBV,the patients with drug-resistant mutation had higher TOS and OSI levels,but lower serum TAS levels(P<0.05).Drug-resistant mutation rate positively correlated with TOS(r= 0.476,P< 0.01) and OSI(r= 0.441,P< 0.01),but negatively correlated with TAS(r=―0.249,P<0.01),except TAS level in patients at HBC group.In addition,the number of mutation sites was positively correlated with disease severity(r=0.614,P<0.01).Conclusion There are different degrees of ox-idative damage in patients with HBV-induced liver disease,and the degree of the damage depends on HBV genotypes and drug-resistant mutations.Therefore,oxidative stress parameters might be useful indicators of progression of HBV-induced liver disease in patients.关键词
乙型肝炎病毒感染/氧化应激/基因型/耐药突变Key words
HBV infection/oxidative stress/genotype/drug-resistant mutation分类
医药卫生引用本文复制引用
杨渝伟,陈小红,彭玲,唐洁,俸家富..HBV感染患者氧化应激状态与病毒基因型和耐药突变的相关性[J].检验医学与临床,2018,15(4):439-443,5.基金项目
国家科技部"863"计划课题(2006AA020905) （2006AA020905）
四川省卫生和计划生育委员会科研课题(130444) （130444）
四川省绵阳市卫生和计划生育委员会科研课题(201221). （201221）
