摘要
Abstract
Objective To study the relationship of miRNA-19a-3p and LDL receptor related protein 2 (LRP2)under hypoxia in rat H9C2 cardiomyocytes.Methods H9C2 cardiomyocytes were treated in hypoxia condition(37 ℃,5% CO2,1% O2)for 0,4,8,12,16,20,24 h.The miRNA-19a-3p level was detected by real time fluorescence quantitative PCR(qRT-PCR)in H9C2 cells and cell culture medium,and the probable target gene LRP2 level were test by ELISA,qRT-PCR and western blot in H9C2 cells and cell culture medium.Re-sults miRNA-19a-3p expression in cell culture medium was much higher under 20 h and 24 h(P<0.05), miRNA-19a-3p expression in H9C2 cells was much lower under 20 h and 24 h(P<0.05).LRP expression in cell culture medium was much higher under 16 h[(675.20 ± 42.40)ng/mL,n=3],20 h[(979.4 ± 204.2)ng/mL, n=3]and 24 h[(1 456.00 ± 363.60)ng/mL,n=3]than 0 h[(245.0 ± 10.84)ng/mL,n=3](P<0.05);the LRP2 mRNA level were much higher in 16 h[(0.000 503 ± 0.000 100)ng/mL,n=3],20 h[(0.001 303 ± 0.000 090)ng/mL,n=3]and 24 h[(0.001 617 ± 0.000 110)ng/mL,n=3](P<0.05);than in the 0 h[(0.000 139 4 ± 0.000 060 0) ng/mL,n=3],the LRP2 protein level were much higher in 20 h[(0.235 000 ± 0.003 427),n= 3] and 24 h [(0.535 000 ± 0.003 427)ng/mL,n=3](P<0.05).Conclusion miRNA-19a-3p may partipate in H9C2 car-diomyocytes necrosis through regulating LRP2.关键词
循环微小RNA-19a-3p/H9C2心肌细胞/缺氧/低密度脂蛋白受体相关蛋白2Key words
miR-19a-3p/H9C2 cardiomyocytes/hypoxia/LDL receptor related protein 2分类
医药卫生
