摘要
Abstract
Objective To analyse blood samples collection,centrifugal time,centrifugal speed,storage time and specimen w hether hemolysis influence factors and detection of plasma prothrombin time(PT),activated partial thromboplastin time(APTT),thrombin time(TT)and fibrinogen(FIB)quantitative four results,which is convenient for daily inspection process control.Methods Retrospective analysis from January 2016 to De-cember 2016 in our hospital laboratory test 859 cases of different factors of coagulation specimens for PT, APTT,TT and Fib four indicators of clinical data.Results PT,APTT and TT results were significantly lon-ger than the qualified samples(P<0.05)were test by blood sampling volume<2.0 mL> and >2.0 mL speci-mens.Fib test results are shorter than the resampling of qualified samples(P<0.05).The results of APTT, TT,PT and Fib were test by 5,10,15 min centrifugation time,and there were no significant differences(P>0.05).There were no statistical differences between the samples centrifugated by 4 000 r/min 5 min and 3 000 r/min 15 min(P>0.05).Specimens at room temperature(20 ℃ to 25 ℃) storage 1 h test after coagulation four results and immediately specimens testing results,there was no significant difference(P>0.05),the spec-imens were stored in 4 h and the APTT was longer than that in 2 h specimens,TT was significantly shorter than that of 2 h specimens(P<0.05);PT and Fib testing results compared with stored 2 h specimens,there were no significant differences(P>0.05).The samples were stored at 4 ℃ environment 1,2,4 h after the tes-ting of coagulation four results,the comparison between different time,there were no significant differences (P>0.05),comparison of different storage time and immediately specimens of four items of blood coagulation detection results,there were no significant differences(P>0.05).Hemolysis tests of PT and APTT were sig-nificantly shorter in hemolysis test results(P<0.05);hemolysis specimens for the detection of T T results sig-nificantly longer than without hemolysis(P<0.05);Fib results of hemolysis and not hemolysis was no signifi-cant difference(P>0.05).Conclusion The inappropriate proportion of anticoagulation,inappropriate storage in room temperature for long time,and hemolysis specimen or not,the causes of the detection accuracy and re-liability of the anomaly.We could use 4 000 r/min centrifugation replacing 3 000 r/min centrifugation for re-porting the detection results to clinicians quicker.关键词
凝血四项/乏血小板血浆/溶血/采血量Key words
four blood coagulation/platelet-poor plasma/hematolysis/blood volume分类
医药卫生
