昆虫学报2017，Vol.60Issue(12)：1411-1419,9.DOI:10.16380/j.kcxb.2017.12.007
泛素-蛋白酶体系统对烟粉虱体内番茄黄曲叶病毒的影响
Effects of ubiquitin-proteasome system on Tomato yellow leaf curl virus in whitefly Bemisia tabaci (Hemiptera: Aleyrodidae)
摘要
Abstract
[Aim] Ubiquitination is an important reversible post-translational modification,which is involved in cell cycle,signal transduction,transcriptional regulation and immune response.However,the function of ubiquitin-proteasome system in virus transmission by insect vectors remains unknown.This study aims to explore the interactions between the ubiquitin-proteasome system of whitefly Bemisia tabaci and Tomato yellow leaf curl virus (TYLCV).[Methods] The expression levels of ubiquitin and proteasome subunit genes in Bemisia tabaci adults feeding on TYLCV-infected and uninfected tomato plants were detected by RT-qPCR.Changes in the contents of free and conjugated ubiquitin proteins in B.tabaci adults after TYLCV infection were detected by Western blotting.After the ubiquitin-proteasome system was disrupted with specific inhibitors or dsRNA injection,changes in the TYLCV content in B.tabaci adults were detected by RT-qPCR.[Results] Carrying TYLCV did not lead to significant change in the expression levels of ubiquitin and 26S proteasome subunit 4,6B and β genes in B.tabaci adults,and had no influence on the contents of free ubiquitin and conjugated ubiquitin proteins and their ratio,suggesting that TYCLV has no effect on the ubiquitin-proteasome system of the whitefly.However,disrupting ubiquitin-proteasome system by specific inhibitors Bortezomib or MG132 or silencing Rprn1 1 by RNAi led to significant increase in the TYLCV content in B.tabaci adults.[Conclusion] Ubiquitinproteasome system negatively regulates the TYLCV content in B.tabaci.Ubiquitin-proteasome system might help the whitefly to counteract the negative influence from TYLCV through degrading the virus directly or activating immune response.关键词
烟粉虱/双生病毒/番茄黄曲叶病毒/病毒传播/泛素/蛋白酶体Key words
Bemisia tabaci/Begomovirus/Tomato yellow leaf curl virus (TYLCV)/virus transmission/ubiquitin/proteasome分类
生物科学引用本文复制引用
夏文强,梁燕,刘银泉,刘树生,王晓伟..泛素-蛋白酶体系统对烟粉虱体内番茄黄曲叶病毒的影响[J].昆虫学报,2017,60(12):1411-1419,9.基金项目
国家重点研发计划(2017YFD0200600,2016YFC1200601) （2017YFD0200600,2016YFC1200601）
现代农业产业技术体系专项(CARS-23-D07) （CARS-23-D07）
