临床神经外科杂志2018，Vol.15Issue(1)：46-49,54,5.DOI:10.3969/j.issn.1672-7770.2018.01.011
高压氧治疗对创伤性脑损伤大鼠的神经功能及CyclinD1表达的影响
Effects of hyperbaric oxygen treatment on neurological functional and expression of CyclinD1 in rats with traumatic brain injury
摘要
Abstract
Objective To investigate the effects of hyperbaric oxygen treatment(HBOT)in rats with traumatic brain injury(TBI)associated to the neurological function improvement and the expression of CyclinD1.Methods According to the random number table,24 adult Sprague Dawley rats were randomly divided into three groups(8 rats each group):sham-operated group,TBI group and HBOT group.The TBI group was subjected to TBI procedure according to the method,the 50 g weight-drop device employed and fallen from 40 cm height to define the severity of the injury;the sham-operated group was subjected to skull open only but not brain injury.The HBOT group was performed for HBO treatment for 10 days(one time every day)after TBI.The neurological severity scores(NSS)records were evaluated after 1,5,10 days post injury.Then all rats were sacrificed and the injury cortex was obtained for the following immunohisto-chemical staining and reverse transcription-polymerase chain reaction(rt-PCR)of CyclinD1.Results Comparing to the shame group,the NSS score records in the TBI group were significantly increased,while HBOT group showed a significant decrease comparing to the TBI rats.Meanwhile,the expressions of protein and mRNA for CyclinD1 in the TBI group were significantly up-regulated compared with the shamed one, while HBOT group was significantly down-regulated compared with TBI rats.Conclusion The HBO treatment ameliorates the neurological function of the rats and inhibits the expression of CyclinD 1 after experimental traumatic brain injury.关键词
创伤性脑损伤/高压氧/细胞周期蛋白D1/神经功能缺损评分/大鼠Key words
hyperbaric oxygen treatment/traumatic brain injury/cyclinD1/NSS/rats分类
医药卫生引用本文复制引用
修光辉,孙洁,李秀玲,尹云玉,李冰沁,凌斌..高压氧治疗对创伤性脑损伤大鼠的神经功能及CyclinD1表达的影响[J].临床神经外科杂志,2018,15(1):46-49,54,5.基金项目
国家自然科学基金(81360289) （81360289）
云南省科技厅-昆明医科大学联合专项基金[2015FB079,2013FB185,2012FB076,2017FE468(-180)] （-180）
