南京医科大学学报（自然科学版）2017，Vol.37Issue(12)：1557-1561,5.DOI:10.7655/NYDXBNS20171204
胃泌素通过JAK2/STAT3信号通路调控胃癌细胞上皮间质转化
Gastrin modulates procedure of epithelial to mesenchymal transition through JAK2/STAT3 pathway in human gastric cancer cells
摘要
Abstract
Objective:To observe the effect of exogenous gastrin-17 on the expression of E-Cadherin and N-Cadherin in human gastric cancer cell line SGC-7901,and discuss the related mechanisms.Methods:SGC-7901 cells were pre-treated with or without YM022 for 1 h and then incubated with G-17 for 30 min to detect the activation of JAK2/STAT3 signaling pathway,for 24 h to detect the expression of E-Cadherin and N-Cadherin,respectively by western blot analysis.SGC-7901 cells were transfected with CCK2R-siRNA or full-length cDNA of human CCK2R (pCMV6-CCK2R),followed by G-17 treatment to detect the expression of p-STAT3,E-Cadherin and N-Cadherin.Cells were pre-treated with AG490 for 1 h or knockdown of STAT3 with siRNA,then incubated with G-17 for 24 h to evaluate the expression of E-Cadherin and N-Cadherin.Results:Western blot assay showed the exogenous G-17 significantly decreased the expression of E-Cadherin and increased the expression of N-Cadherin,meanwhile,activated the JAK2/STAT3 signaling pathway.Specific antagonist or siRNA against CCK2R partly blocked gastrin-induced activation of STAT3 and the expression of E-Cadherin and N-Cadherin,which suggested the effect of gastrin was CCK2R dependent.Specific inhibitor of JAK2/STAT3 signaling pathway AG490 and siRNA against STAT3 partly attenuated the effect of gastrin on the expression of E-Cadherin and N-Cadherin.Conclusion:Gastrin acting on the cholecystokinin2 receptor,down-regulates expression of E-Cadherin and up-regulates the expression of N-Cadherin,via activation of JAK2/STAT3 signaling pathway induced EMT in human gastric cancer cells.关键词
胃泌素/胃癌/JAK2/STAT3信号转导通路/上皮间质转化Key words
gastrin/gastric cancer/JAK2/STAT3 signaling pathway/epithelial to mesenchymal transition分类
医药卫生引用本文复制引用
罗振国,朱国琴,许海尘,徐伟..胃泌素通过JAK2/STAT3信号通路调控胃癌细胞上皮间质转化[J].南京医科大学学报（自然科学版）,2017,37(12):1557-1561,5.基金项目
江苏省自然科学基金(BK20151038) （BK20151038）
