山东医药2018，Vol.58Issue(4)：1-4,4.DOI:10.3969/j.issn.1002-266X.2018.04.001
白血病抑制因子受体在宫颈癌肺转移过程中的作用及其机制
Effects of leukaemia inhibitory factor receptor on lung metastasis of cervical cancer
摘要
Abstract
Objective To investigate the role of leukemia inhibitory factor receptor(LIFR)and Hippo-YAP signaling pathway in the lung metastasis of cervical cancer.Methods ① The real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression of LIFR mRNA in the primary cancerous tissues,adjacent non-can-cerous tissues and metastasis tissues from 50 cases of patients with cervical cancer and lung metastasis.② Recombinant lentiviral plasmid containing LIFR or its negative control(vector)were used to infect the human cervical cancer cell line SiHa.After screening with 2.0 μg/mL puromycin, the cells that stably expressed LIFR(observation group)or vector (control group)were obtained.Western blotting was used to detect the expression of LIFR and Hippo-YAP signaling path-way related proteins YAP, P-YAP, TAZ, and p-TAZ.The cells of the two groups were injected into the BALB /c nude mice through the tail vein.After 4 weeks,the nude mice were killed and the lung tissue was removed for HE staining.The number of all the metastases in the lung tissues was counted microscopically.Results ①The relative expression of LIFR mRNA in the primary tumor was higher than that in the adjacent non-cancerous tissues,and the relative expression of LIFR mRNA in the metastasis tissues was lower than that in primary tumor and adjacent non -cancerous tissues(all P<0.05).②The relative expression of LIFR,p-YAP and p-TAZ proteins in the SiHa cells which overexpressed LIFR was higher than that in the control cells(all P<0.05).There was no significant difference in YAP and TAZ protein expression between the control group and observation group(all P>0.05).The lung metastases in the SiHa cells with overexpressed LIFR and control cells were 9 ±2 and 60 ±5, respectively(all P<0.05).Conclusion Overexpression of LIFR can promote the phosphorylation of Hippo-YAP signaling pathway proteins YAP and TAZ,and then inhibit the formation of lung metastasis of cervical cancer cells.关键词
宫颈癌/肺转移/白血病抑制因子受体/白血病抑制因子/河马-转录共激活因子YES相关蛋白Key words
cervical carcinoma/lung metastasis/leukaemia inhibitory factor receptor/leukaemia inhibitory factor/Hippo-YAP分类
医药卫生引用本文复制引用
贺丹,刘海凤,蒋欣妮..白血病抑制因子受体在宫颈癌肺转移过程中的作用及其机制[J].山东医药,2018,58(4):1-4,4.基金项目
四川省卫生厅科研课题(130303). （130303）
