山东医药2018，Vol.58Issue(4)：5-7,3.DOI:10.3969/j.issn.1002-266X.2018.04.002
HLA-G基因修饰的胎盘间充质干细胞对C D4+T淋巴细胞增殖的影响
Effect of placenta-derived mesenchymal stem cells modified by HLA-G on proliferation of CD4 +T lymphocytes
摘要
Abstract
Objective To investigate the effect of placenta-derived mesenchymal stem cells modified by human leuko-cyte antigen G(HLA-G)on the proliferation of CD4 +T lymphocytes.Methods Placenta-derived mesenchymal stem cells from newborn were cultured and identified.Placenta mesenchymal stem cells in logarithmic phase were randomly divided into the PEGFP-N1-HLA-G group,PEGFP-N1 group,and the control group.The former two groups were separately trans-fected with PEGFP-N1-HLA-G plasmid and empty vector PEGFP-N1.The control group was cultured conventionally.After culturing for 20 h,HLA-G protein was detected.The results suggested that PEGFP-N1-HLA-G plasmid promoted the ex-pression of HLA-G protein,and the transfection was successful.In vitro, CD4 +T lymphocytes were isolated from healthy people peripheral blood and mixed with the cells in each group.After culturing for 24,48 and 72 h,the inhibition rate of CD4 +T lymphocyte growth was detected by MTT assay,respectively.Results The proliferation inhibition rate of CD4 +T lymphocytes in the PEGFP-N1-HLA-G group was higher than that in the control group and PEGFP-N1 group at 24,48,and 72 h after co-culture(all P<0.05).Conclusion The placental mesenchymal stem cell modified by HLA-G gene can in-hibit the proliferation of CD4 +T lymphocytes.关键词
胎盘间充质干细胞/人类白细胞抗原G/T淋巴细胞/细胞增殖Key words
placental mesenchymal stem cells/human leukocyte antigen G/T lymphocytes/cell proliferation分类
医药卫生引用本文复制引用
崔桂玉,白剑,苗兰英,林大勇..HLA-G基因修饰的胎盘间充质干细胞对C D4+T淋巴细胞增殖的影响[J].山东医药,2018,58(4):5-7,3.基金项目
国家自然科学基金面上项目(81571555) （81571555）
辽宁省自然科学基金资助项目(2015020714). （2015020714）
