山东医药2018，Vol.58Issue(4)：8-11,4.DOI:10.3969/j.issn.1002-266X.2018.04.003
αGVHD小鼠靶器官组织TLR4/MyD88/NF-κB信号通路蛋白表达变化及意义
Expression changes of TLR4/MyD88/NF-κB signaling pathway in target organs of mice with acute graft-versus-host disease
摘要
Abstract
Objective To investigate the expression changes of Toll-like receptor-4(TLR4)/myeloid differentiation primary response gene 88(MyD88)/nuclear factor-κB(NF-κB)signaling pathway protein in mice with acute graft-versus-host disease(aGVHD)after allogeneic bone marrow transplantation(allo-BMT).Methods Allogeneic bone marrow trans-plantation was performed with C57BL/6 male mice as donors and BALB/C female mice as recipients.The aGVHD models of mice(aGVHD group)were established by injecting the donor bone marrow cells and splenic lymphocytes after the recipients were treated with the lethal dose preconditioning regimen of Busulfan +cyclophosphamide.Syngeneic bone marrow transplan-tation(Syn-BMT)was performed with BALB/C male mice as donors and BALB/C female mice as recipients.The bone mar-row cells from donor mice were injected into the recipient mice after equal dose of preconditioning regimen,and the syngeneic bone marrow transplantation model was established as the control group.On the 14 day after transplantation,5 survival mice were taken from the aGVHD group and the control group,respectively.The liver, intestine, spleen, and skin tissues were taken from the mice.Real-time quantitative polymerase chain reaction(qRT-PCR), Western blotting, and immunohisto-chemistry were used to detect the mRNA and protein expression rates of TLR 4,MyD88,NF-κB p65 and p50 in the target or-gans of mice.Results The mRNA expression and protein expression,and the the protein expression rates of TLR4,MyD88, NF-κB p65 and p50 in the liver,intestine,spleen,and skin tissues of the GVHD group were higher than those of Syn-BMT control group(all P<0.05).Conclusion The expression of TLR4/MyD88/NF-κB signaling pathway protein in the liver, intestine,spleen and skin of aGVHD model mice is up-regulated at the gene level and protein level, suggesting that the TLR4/MyD88/NF-κB signaling pathway may be involved in the pathogenesis of aGVHD after allo-BMT.关键词
急性移植物抗宿主病/异基因骨髓移植/Toll样受体4/髓样分化蛋白88/核转录因子κBKey words
acute graft-versus-host disease/allogeneic bone marrow transplantation/Toll-like receptor-4/myeloid differentiation primary response gene 88/nuclear factor-κB引用本文复制引用
江洋洋,涂三芳,吴远彬,李玉华..αGVHD小鼠靶器官组织TLR4/MyD88/NF-κB信号通路蛋白表达变化及意义[J].山东医药,2018,58(4):8-11,4.基金项目
国家自然科学基金面上项目(81372249) （81372249）
广东省科技计划项目(2016A020213005) （2016A020213005）
广东省中国科学院全面战略合作专项资金竞争性分配项目(2013B091500072). （2013B091500072）
