山东医药2018，Vol.58Issue(4)：17-20,4.DOI:10.3969/j.issn.1002-266X.2018.04.005
HSP70小干扰RNA对人瘢痕成纤维细胞胶原表达的影响
Effects of HSP70 siRNA on collagen expression in the human keloid fibroblasts
摘要
Abstract
Objective To investigate the effects of heat shock protein 70(HSP70)small interfering RNA(HSP70 siRNA)on the collagen production of the human keloid fibroblasts.Methods Collecting 56 cases of human keloid tis-sues,isolating the human keloid fibroblasts and culturing them in vitro.The human keloid fibroblasts were divided into the observation group,the negative group and the blank control group.The HSP70 siRNA and the negative siRNA were trans-fected into the cells of the observation group and the negative group by the liposome, respectively.Moreover, the blank control group was not transfected.At 24 h after the transfection,the mRNA expression levels of HSP70,collagen Ⅰ,and collagen Ⅲwas detected by the real-time PCR.The protein expression levels of HSP70,collagen I,and collagen Ⅲwere detected by Western blotting.The total collagen in the medium was analyzed by the Sircol collagen assay.Results Com-pared with the negative group and the blank control group,the mRNA and protein expression levels of HSP 70,collagenⅠ, and collagen Ⅲdecreased in the observation group,as well as the total collagen in the medium(all P<0.05).However, there were no differences between the negative group and the blank control group(all P>0.05).Conclusion Transfect-ing the HSP70 siRNA could inhibit the collagen expression in the human keloid fibroblasts.关键词
热休克蛋白70/RNA干扰/小干扰RNA/人瘢痕成纤维细胞/胶原蛋白Key words
heat shock protein 70/RNA interference/siRNA/human keloid fibroblasts/collagen分类
医药卫生引用本文复制引用
王杨杨,李小静..HSP70小干扰RNA对人瘢痕成纤维细胞胶原表达的影响[J].山东医药,2018,58(4):17-20,4.基金项目
安徽省科技计划项目(KJ2015A114). （KJ2015A114）
