陕西中医2018，Vol.39Issue(2)：139-142,4.DOI:10.3969/j.issn.1000-7369.2018.02.001
"嗅三针"对阿尔茨海默病小鼠空间记忆能力和海马APP、Aβ蛋白表达的影响
Influence of"Xiusanzhen"electroacupuncture on spatial memory ability and APP、Aβ protein expression in hippocamus of mice with Alzheimer's disease
摘要
Abstract
Objective:To observe the intervening effect of"Xiusanzhen"Electroacupuncture(EA)on spatial memory ability and APP、Aβ protein expression in hippocampus of mice with Alzheimer's disease(AD),in order to clarify its therapeutic mechanism based on the olfactory nerve pathway in alleviating AD.Method:40 AD mice were randomly devided into 4 groups:AD model group,"Xiusanzhen"EA group,"Xiusanzhen"EA with olfactory nerve transected group(ONT+EA),Donepezil HCl group,with 10 mice respectively in each group,then took 10 negative mice as normal control group.Electroacupuncture intervention of"Xiusanzhen"was applied to stimulate bilateral LI 20 and EX-HN3,intragastric administration of donepezil HCI in AD mice was performed at the dose of 3 mg/(kg· d),once daily intervention(except weekends)for 4 weeks in all.Morris water maze was applied to test spatial memo-ry ability of mice.Western blot was applied to test APP、Aβ protein expression in hippocampus of mice.Results:"Xiusanzhen"EA group and Donepezil HCl group could increase number of traversing platforms and target quadrant percent of total time,meanwhile,reduce APP、Aβ protein expression in hippocampus of AD mice(P<0.05);ONT+EA group had no significant differences in influencing indexes of AD mice(P>0.05),there were no significant difference between"Xiusanzhen"EA group and donepezil HCL group(P>0.05).Conclusion:"Xiusanzhen"EA has the effect in ameliorating spatial memory ability and reducing APP、Aβ protein expression in hippocampus of mice with AD,the integrity of the olfactory pathway is the core mechanism of the intervention effect.关键词
阿尔茨海默病/针灸疗法/@嗅三针/海马/动物实验/小鼠Key words
Alzheimer disease/acupuncture-moxibustion @ Xiusanzhen Hippocampus Animal experi-mentation Mice分类
医药卫生引用本文复制引用
王渊,刘智斌,牛文民,王强,李杰,鲁刚.."嗅三针"对阿尔茨海默病小鼠空间记忆能力和海马APP、Aβ蛋白表达的影响[J].陕西中医,2018,39(2):139-142,4.基金项目
国家自然科学基金资助项目(81503667)陕西中医药大学重点培育科研项目(2015PY07) （81503667）
