陕西中医2018，Vol.39Issue(2)：150-153,4.DOI:10.3969/j.issn.1000-7369.2018.02.004
黄芩素对肝癌细胞HepG2放疗增敏作用的机制研究
Study on the mechanism of enhancing the sensitivity of baicalein to radiotherapy of hepatoma cell line HepG2
摘要
Abstract
Objective:Investigate the mechanism of baicalein in radiosensitization,and to provide a new strate-gy for radiosensitization of liver cancer.Methods:HepG2cells were divided into control group,radiotherapy group, drug group,drug plus radiotherapy group,liver cells plus drug group,the formation rate of cell proliferation was detected using MTT activity,flow cytometry to detect the apoptosis,cell clone detection,and use the western blot-ting method to detect the level of p53 and QRT-PCA.Results:Drugs plus radiotherapy group HepG2cell survival rate was significantly lower than that of other groups(P<0.05);drug plus radiotherapy group HepG2cell colony formation and clone formation rate was significantly lower than that of other groups(P<0.05),while still signifi-cantly higher than liver cells plus drug group(P<0.05);drug plus radiotherapy group the apoptosis rate of HepG2 cells was significantly higher than that of other groups;drugs HepG2plus radiotherapy group p-53/beta cell-actin protein level was significantly higher than that of other groups;drug plus radiotherapy group p-53/beta-actin mR-NA HepG2cells was significantly higher than other groups.Conclusion:Baicalein can significantly increase the sensi-tivity of radiation therapy,increase the apoptosis rate of HepG2cells,and effectively regulate the levels of p53 pro-tein and mRNA.关键词
肝肿瘤/中西医结合疗法/黄芩/治疗应用/HepG2细胞/放射疗法Key words
Liver neoplasms/integrated Chinese traditional and western medicine therapy Scutellaria ba-icalensis/therapeutic uses Hep G2 Cells Radiotherapy分类
医药卫生引用本文复制引用
安鹏,李星瑶,蔡子墨,党慧敏,吴喜利..黄芩素对肝癌细胞HepG2放疗增敏作用的机制研究[J].陕西中医,2018,39(2):150-153,4.基金项目
陕西省科学技术研究发展计划项目(国际合作,2013KW-26-03)陕西省中医药管理局科研项目(JC38) （国际合作,2013KW-26-03）
