同济大学学报（医学版）2017，Vol.38Issue(6)：1-5,11,6.DOI:10.16118/j.1008-0392.2017.06.001
微RNA对脊髓损伤小鼠恢复的影响
Effect of microRNAs on recovery of spinal cord injury in mice
摘要
Abstract
Objective To investigate the effect of microRNAs on the recovery of spinal cord injury in mice.Methods Target genes and miRNAs were searched and predicted,the results were confirmed in 293T cells.Spinal cord injury model was constructed in mice.The lentivirus-mediated miRNAs were injected via tail vein in mice with spinal cord injury.BBB behavioral experiment was performed and the morphology of spinal cord was observed.The expression of markers and target genes were detected.Results The miR-9,miR-124 and miR-125 induced the down-regulation of three or more target genes in vitro.miR-9 and miR-124 promoted the recovery of spinal cord injury caused by clamping;miR-9 group was better on movement function recovery,however,the effect of miR-124was worse than that of control group.In miR-9 group,GFAP was down-regulated,MAP2 and NeuN were up-regulated,and the target genes GLIS3 and CYBRD1 were also down-regulated.Conclusion MiR-9 may promote the repair of spinal cord injury by down-regulating the expression of target genes GLIS3 and CYBRD1 in mice with spinal cord injury.关键词
微RNA/脊髓损伤/细胞转分化Key words
miRNA/spinal cord injuries/cell transdifferentiation分类
医药卫生引用本文复制引用
孟必成,朱小奇,李琛,刘海亮..微RNA对脊髓损伤小鼠恢复的影响[J].同济大学学报（医学版）,2017,38(6):1-5,11,6.基金项目
国家“九七三”重点基础研发计划(2012CB966903) （2012CB966903）
国家自然科学基金(31671539) （31671539）
上海市科委基础研究重点项目(13JC1407102) （13JC1407102）
