同济大学学报（医学版）2017，Vol.38Issue(6)：12-17,6.DOI:10.16118/j.1008-0392.2017.06.003
人不同组织间充质干细胞免疫调控能力的比较
Comparison of immune regulatory properties of human mesenchymal stem cells derived from different tissues
摘要
Abstract
Objective To compare the immunomodulatory properties of human mesenchymal stem cells (MSCs) from different tissues.Methods Human adipose tissue-derived MSCs,umbilical cordderived MSCs,bone marrow-derived MSCs,dental pulp-derived MSCs and skin-derived MSCs were treated with IFNγ and TNFα,then co-cultured with peripheral blood mononuclear cells (PBMCs).The proliferation of PBMCs was detected;and gene expression of PBMCs and MSCs was detected by RT-PCR and Western blotting.Results The proliferation of PBMCs co-cultured with stimulated MSCs was significantly inhibited.There were no significant differences in the inhibiting effects on PBMCs among MSCs from five different tissues.Conclusion MSCs derived from human adipose,umbilical cord,bone marrow,dental pulp and skin have no differences in immunomodulatory effect on mononuclear cells.关键词
免疫调控/间充质干细胞/单个核细胞/吲哚胺-2,3-双加氧酶/趋化因子Key words
immunoregulation/mesenchymal stem cells/peripheral blood mononuclear cells/indoleamine-2,3-dioxygenase/chemokines分类
医药卫生引用本文复制引用
刘贤俊,刘海亮,景波,张春雪,孙毅..人不同组织间充质干细胞免疫调控能力的比较[J].同济大学学报（医学版）,2017,38(6):12-17,6.基金项目
国家“九七三”重点基础研究发展计划(2014CB964602、2016YFA0100801) （2014CB964602、2016YFA0100801）
