同济大学学报（医学版）2017，Vol.38Issue(6)：18-22,5.DOI:10.16118/j.1008-0392.2017.06.004
脓毒症肺损伤小鼠血清内皮微颗粒的变化
Circulating CD144 + endothelial-derived microparticles in mice with sepsis-induced acute lung injury
摘要
Abstract
Objective To investigate the changes of circulating CD144+ endothelial-derived microparticles(EMPs) in mice with sepsis-induced acute lung injury.Methods Sepsis model was induced by cecal ligation and puncture(CLP) in C57BL/6J mice,the mice with sham operation were used as control group.The blood and lung tissue samples of survived mice were collected 24 h after operation.The endothelial-derived microparticles (EMPs)were isolated by differential centdfugation and determined by flow cytometry;serum IL-1β,IL-6 and TNF-α levels were measured by ELISA;lung tissue wet-to-dry weight ratio was calculated.The histopathological changes and ultrastmcture of lung tissue were observed with lightmicroscopy (HE stain) and scanning electron microscopy,respectively.Results The lung wet/dry ratios (W/D),serum IL-1β,IL-6,TNF-α and EMPs in CLP group were significantly higher than those in sham group (all P ＜ 0.01).Histopathological observation showed that the structure of lung tissue in sham group appeared normal,while the shrinks of alveolar and disappearance of alveolar architecture were found in CLP group.Electron microscopy showed edema and thickening of alveolar wall in CLP group,while the alveolar ultrastucture was normal in sham group.Conclusion The sepsis-induced lung injuryoccurs aftercecal ligation and puncturein mice.The increased EMPs are closely related to sepsis-induced lung injury,indicating EMPs may be involved in the pathological process.关键词
脓毒症/急性肺损伤/内皮微颗粒/小鼠Key words
endothelial-derived microparticles/acute lung injury/sepsis/mice分类
医药卫生引用本文复制引用
殷颖杰,章守琴,王三强,高成金,张翔宇..脓毒症肺损伤小鼠血清内皮微颗粒的变化[J].同济大学学报（医学版）,2017,38(6):18-22,5.基金项目
上海市自然科学基金(15ZR1433100) （15ZR1433100）
