同济大学学报（医学版）2017，Vol.38Issue(6)：27-32,6.DOI:10.16118/j.1008-0392.2017.06.006
聚二恶烷酮气管支架的动物实验研究
Feasibility and biodegradability of polydioxanone tracheal stent in rabbits
摘要
Abstract
Objective To study the feasibility and biodegradability of polydioxanone (PDO) tracheal stent in rabbits.Methods The mechanical properties of laboratory-made PDO tracheal stents were measured in vitro and compared with those of tracheas from 6 healthy rabbits.The PDO tracheal stents were surgically implanted into the cervical tracheas of 20 healthy rabbits.The rabbits were euthanized at 4,8,12 weeks after implantation.The biodegradability and tissue response were observed by bronchoscopy,histology and scanning electron microscopy.Six healthy rabbits underwent sham surgery without PDO tracheal implantation as control group.Results The average radial supporting force and elastic restoring rate of the rabbit cervical tracheas in vitro were 157.8 cN and 93.42％,respectively;those of PDO tracheal stents with the diameter of 6 mm were 192.43 cN and 96.8％,respectively.The majority of the 20 experimental rabbits implanted with PDO tracheal stents had decreased appetite in the short term.One rabbits had cervix pneumoderma;4 rabbits had mucopurulent nasal discharge;5 rabbits had intermittent stridor.Two rabbits died of airway obstruction due to secretions at 1 and 2 weeks after surgery,respectively;and autopsy revealed that two rabbits had pulmonary infection with complete expansion of the stents.The appetite and activity of the survival rabbits returned to normal at 4 weeks after operation.The rabbits in the control group showed no abnormal symptoms after operation.Four weeks after operation,the tracheal stents were integrated without displacement,and the lumens were in the open state with different degrees of secretion coverage on the of tracheal stents;bronchial mucosa covered with airway secretions and inflammatory cells,and the PDO fiber was integrated with broken fiber surface.Eight weeks after operation,the tracheal stents were well outlined with new tissue inclusions.The airway secretions of bronchial mucosa and inflammatory cells decreased significantly,and the PDO fiber appeared cracking and partly absorbing.Twelve weeks after operation,the tracheal stents disappeared morphologically,and there was residual and thick tissue in the lumens.The PDO fiber showed completely absorption and residual PDO fragments were found on the bronchial mucosa.Conclusion The biodegradable PDO stent exhibits mechanical strength comparable to that of rabbit cervical trachea in vitro and has good biodegradability,suggesting that it can play the role of expansion and support in healthy rabbit trachea.关键词
气管支架/聚二恶烷酮/生物可降解Key words
tracheal stent/polydioxanone/biodegradability分类
医药卫生引用本文复制引用
阮征,王晋,章晓淼,李毓陵,傅本胜..聚二恶烷酮气管支架的动物实验研究[J].同济大学学报（医学版）,2017,38(6):27-32,6.基金项目
上海市卫生和计划生育委员会面上项目(20134317) （20134317）
