水生态学杂志2018，Vol.39Issue(1)：83-90,8.DOI:10.15928/j.1674-3075.2018.01.013
IHB系稀有鮈鲫遗传结构研究
Genetic Structure of IHB Rare Minnow( Gobiocypris rarus)
摘要
Abstract
The rare minnow ( Gobiocypris rarus) is a cyprinid species endemic to the upper Yangtze River, charac-terized by small size, early sexual maturation, consecutive spawning and sensitivity to chemicals.The species has been widely used as a test organism in environmental science, genetics, ichthyopathology and ecotoxicology.How-ever, wild G.rarus populations are threatened by human activities and decreasing rapidly.The IHB rare minnow is a closed laboratory animal colony, the offspring of 50 wild G.rarus specimens collected in Hanyuan County of Si-chuan Province in 2006 and bred using methods that prevent inbreeding.In 2014, the ninth generation (F9) of the original parent generation ( P0) was obtained.In this study, 12 microsatellite loci were used to reveal the genetic structure of the IHB rare minnow from ( P0 ) to ( F8 ) , aiming to evaluate the genetic quality of the IHB rare min-now, provide a reference for the construction and maintenance of a closed laboratory animal colony and support ef-forts to protect the species.As breeding proceeded from generation P0 to F8, the number of alleles per locus and average effective number of alleles per locus decreased, respectively, from 4.4 to 4.1 and 4.0 to 3.0.The average expected heterozygosity and polymorphic information content also decreased, from 0.5878 to 0.5518 and 0.5509 to 0.5293.The largest Nei genetic distance (0.0702) and smallest similarity coefficient (0.9298) were between P0 and F8 and the smallest genetic distance (0.0078) and the largest similarity coefficient (0.9937) were between F7 and F8.These findings indicates that the genetic distance and genetic differentiation ( Fst) increased between the original generation and the last generation, but decreased between successive generations.Six allelic genes were lost during development of the IHB colony but no significant changes were found in gene frequencies after the 5th generation.Although genetic diversity declined slightly, a relatively high and acceptable level was maintained in the IHB colony and inbreeding was controlled effectively.Therefore, the genetic structure of IHB colony is stable and the colony meets the genetic quality control requirement for laboratory animals.The IHB colony can be used as a laboratory animal and as an in vivo gene pool for the topotype population of G.rarus.关键词
IHB系稀有鮈鲫/实验动物封闭群/遗传质量控制/微卫星DNA标记/遗传结构Key words
IHB rare minnow/closed laboratory animal colony/genetic quality control/microsatellite marker/ge-netic structure分类
生物科学引用本文复制引用
李慧慧,王春伶,王剑伟..IHB系稀有鮈鲫遗传结构研究[J].水生态学杂志,2018,39(1):83-90,8.基金项目
国家科技支撑计划(2011BAI15B01-41) （2011BAI15B01-41）
湖北省科技支撑计划2015BCE098. （）
