水生态学杂志2018，Vol.39Issue(1)：105-110,6.DOI:10.15928/j.1674-3075.2018.01.016
患病蝶尾金鱼维氏气单胞菌温和生物变种的分离鉴定
Isolation, Identification and Antibiotic Sensitivity Analysis of Aeromonasveronii Biovar Sobria from Diseased Butterfly Tail Goldfish
摘要
Abstract
Aeromonas veronii has recently become a serious threat to aquaculture and healthy fish farming.Fish in-fected by Aeromonas spp.often display hemorrhagic septicemia, resulting in high mortality and severe economic loss.However, there are few reports about the isolation and identification of pathogens isolated from butterfly tail goldfish infected with Aeromonas veronii.In this study, the morphological, physiological and biochemical character-istics of an Aeromonas veronii strain isolated from the goldfish was analyzed.Amplification of the bacterial small subunit ribosomal DNA (16S rDNA) gene sequence was carried out using the polymerase chain reaction ( PCR) technique using universal primers for bacteria.A bacterial strain was isolated from the liver of the diseased goldfish and named DW-1.The strain DW-1 is a short, gram-negative bacillus that produces gas from glucose and tested positive for oxidase, lysine decarboxylase, indole, nitrate reduction, gluconate and methyl red ( MR) , while tests were negative for ornithine decarboxylase, urease, phenylalaninase, citrate, hydrogen sulfide and Vogues Proskauer (VP).A 1385 bp DNA fragment was obtained upon PCR amplification of the 16S rDNA gene.Phylogenetic tree analysis shows that the strain is most closely related to Aeromonas veronii biovar sobria, strain ATCC9071, and ho-mology of the 16S rDNA sequences was 99%-100%.Thus, the DW-1 strain was positively identified as Aero-monas veronii biovar sobria.Animal infection experiments indicated that the mortality was 100% to zebrafish and the median lethal dose ( LD50 ) was 1.17 ×106 CFU/mL.Antibiotic sensitivity results show that the isolated strain was sensitive to 23 antibiotics including cefixime, cefoperazone, cefotaxime, cephalexin, streptomycin, kanamy-cin, norfloxacin, enrofloxacin, levofloxacin, vancomycin, azithromycin, gentamicin, neomycin, amikacin, chlor-amphenicol, florfenicol and meropenem, but was resistant to imipenem, nalidixic acid, enoxacin, amoxicillin, am-picillin, rifampicin and tetracycline.The study provides a scientific reference for the prevention and treatment of bacterial diseases in goldfish.关键词
蝶尾金鱼/维氏气单胞菌/分离鉴定/药敏试验Key words
goldfish butterfly/Aeromonas veronii/isolation and identification/antibiotic sensitivity test分类
农业科技引用本文复制引用
胡秀彩,孙金辉,孙敬锋,宋亚娇,吕爱军,陈成勋..患病蝶尾金鱼维氏气单胞菌温和生物变种的分离鉴定[J].水生态学杂志,2018,39(1):105-110,6.基金项目
天津市自然科学基金重点项目(16JCZDJC33500,15JCZDJC34000) （16JCZDJC33500,15JCZDJC34000）
国家自然科学基金(31272692) （31272692）
天津市水产产业技术体系创新团队(ITTFRS2017009) （ITTFRS2017009）
河南省高等学校重点科研项目(17A240001). （17A240001）
