生态学报2018，Vol.38Issue(2)：615-623,9.DOI:10.5846/stxb201608231718
氮磷添加对杉木林土壤碳氮矿化速率及酶动力学特征的影响
Effects of nitrogen and phosphorus additions on soil organic carbon and nitrogen mineralization and hydrolase kinetics in Chinese fir plantations
摘要
Abstract
A long-term field experiment of nitrogen (N) and phosphorus (P) additions was established in Chinese fir plantations in subtropical China.Four treatments,i.e.control (CK),N (50kg N hm-2 a-1),P (50kg P hm-2 a-1),and NP (50kg N hm-2 a-1+ 50kg P hm-2 a 1),were selected in this study.We studied the effects of N and P additions on the soil mineralization rate of carbon (Cmin) and nitrogen (Nmin) and the kinetics of β-glucosidase (βG) and N-acetyl-glucosaminidase(NAG).The results showed that (1) N additions had negative effects on Cmin and N which were lower by 25％ and 18％,respectively,compared to CK.(2) P (P,NP) additions decreased the Vmax and Km of NAG by 26％-60％ compared to CK,whereas NP combined additions greatly enhanced the catalytic efficiency (Vmax/Km) of βG and NAG (P＜0.05).(3) Cmin was positively correlated with dissolved organic carbon (DOC) contents,whereas Nmin was positively correlated with pH and negatively correlated with NH4+-N and NO3--N contents.The catalytic efficiencies of βG and NAG were negatively correlated with NH4+-N and NO3--N contents (P＜0.05).The Km of βG and NAG was positively correlated with NH4+-N and NO3--N (P＜0.05),The Vmax of βG was positively correlated with NH4+-N and NO3--N (P＜0.05),and the V of NAG was negatively correlated with soil organic carbon,total nitrogen,total phosphorus,and available contents (P＜ 0.05).Our results suggest that N additions had negative effects on Cmin and Nmin by decreasing soil pH,increasing soil available N contents,and inhibiting the Vmax/Km of βG and NAG,whereas NP additions increased soil available P and the Vmax/Km of βG and NAG in southern subtropical Chinese fir plantations.关键词
氮磷添加/碳氮矿化/酶动力学/水解酶/亚热带红壤Key words
nitrogen and phosphorus additions/soil carbon and nitrogen mineralization/enzyme kinetics/Hydrolase/subtropical red soil引用本文复制引用
聂二旗,张心昱,郑国砥,杨洋,王辉民,陈伏生,孙晓敏..氮磷添加对杉木林土壤碳氮矿化速率及酶动力学特征的影响[J].生态学报,2018,38(2):615-623,9.基金项目
国家自然科学基金项目(41571251,41571130043) （41571251,41571130043）
中科院技术创新项目(201604) （201604）
